PMID: 9420209Jan 7, 1998Paper

The papillomavirus minor capsid protein, L2, induces localization of the major capsid protein, L1, and the viral transcription/replication protein, E2, to PML oncogenic domains

Journal of Virology
P M DayJohn T Schiller

Abstract

We have used immunofluorescent staining and confocal microscopy to examine the subcellular localization of structural and nonstructural bovine papillomavirus (BPV) proteins in cultured cells that produce infectious virions. When expressed separately, L1, the major capsid protein, showed a diffuse nuclear distribution while L2, the minor capsid protein, was found to localize to punctate nuclear regions identified as promonocytic leukemia protein (PML) oncogenic domains (PODs). Coexpression of L1 and L2 induced a relocation of L1 into the PODs, leading to the colocalization of L1 and L2. The effect of L2 expression on the distribution of the nonstructural viral proteins E1 and E2, which are required for maintenance of the genome and viral DNA synthesis, was also examined. The localization of the E1 protein was unaffected by L2 expression. However, the pattern of anti-E2 staining was dramatically altered in L2-expressing cells. Similar to L1, E2 was shifted from a dispersed nuclear locality into the PODs and colocalized with L2. The recruitment of full-length E2 by L2 occurred in the absence of other viral components. L2 was shown previously to be essential for the generation of infectious BPV. Our present results provide evidence...Continue Reading

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Citations

Sep 27, 2000·The Journal of Biological Chemistry·D RangasamyV G Wilson
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P LenzJ T Schiller
Sep 5, 2002·Journal of Virology·Luise FlorinMartin Sapp
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Mar 1, 2003·Journal of Virology·Rongcun YangRichard B S Roden
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patricia M DayJohn T Schiller
Jun 28, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A González Martín
Nov 22, 2013·Journal of Virology·Melanie SchmidRamon A Gonzalez

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