The paralogous MarR/DUF24-family repressors YodB and CatR control expression of the catechol dioxygenase CatE in Bacillus subtilis.

Journal of Bacteriology
Bui Khanh ChiHaike Antelmann

Abstract

The redox-sensing MarR/DUF24-type repressor YodB controls expression of the azoreductase AzoR1 and the nitroreductase YodC that are involved in detoxification of quinones and diamide in Bacillus subtilis. In the present paper, we identified YodB and its paralog YvaP (CatR) as repressors of the yfiDE (catDE) operon encoding a catechol-2,3-dioxygenase that also contributes to quinone resistance. Inactivation of both CatR and YodB is required for full derepression of catDE transcription. DNA-binding assays and promoter mutagenesis studies showed that CatR protects two inverted repeats with the consensus sequence TTAC-N(5)-GTAA overlapping the -35 promoter region (BS1) and the transcriptional start site (TSS) (BS2). The BS1 operator was required for binding of YodB in vitro. CatR and YodB share the conserved N-terminal Cys residue, which is required for redox sensing of CatR in vivo as shown by Cys-to-Ser mutagenesis. Our data suggest that CatR is modified by intermolecular disulfide formation in response to diamide and quinones in vitro and in vivo. Redox regulation of CatR occurs independently of YodB, and no protein interaction was detected between CatR and YodB in vivo using protein cross-linking and mass spectrometry.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·Journal of Bacteriology·M WetzsteinW Schumann
Jan 1, 1992·Toxicology and Applied Pharmacology·T J MonksD G Graham
Jan 1, 1991·Chemico-biological Interactions·P J O'Brien
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Apr 16, 2002·Chemical Research in Toxicology·Yoshito KumagaiNobuhiro Shimojo
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mayuree Fuangthong, John D Helmann
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shunji NakanoPeter Zuber
Oct 1, 2005·Molecular Microbiology·Falko HochgräfeMichael Hecker
Jun 15, 2006·Antioxidants & Redox Signaling·Lars I Leichert, Ursula Jakob
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jin-Won LeeJohn D Helmann
Jul 6, 2007·The Journal of Biological Chemistry·Falko HochgräfeMichael Hecker
Feb 20, 2008·Expert Review of Proteomics·Haike AntelmannPeter Zuber
Aug 5, 2008·Molecular Microbiology·Manuel LiebekeHaike Antelmann
Jul 7, 2009·Nature Chemical Biology·Gerald L NewtonRobert C Fahey
Oct 20, 2009·Journal of Bacteriology·Dierk-Christoph PötherMichael Hecker

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Citations

Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Quanjiang JiChuan He
Jan 13, 2012·Nucleic Acids Research·Gottfried J PalmHaike Antelmann
Mar 19, 2013·Journal of Bacteriology·Semen A LeynDmitry A Rodionov
Aug 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Sang Jae LeeBong-Jin Lee
Jul 4, 2017·Critical Reviews in Biochemistry and Molecular Biology·Dinesh K Deochand, Anne Grove
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Sep 13, 2017·Antimicrobial Agents and Chemotherapy·Anna KnöppelDan I Andersson
Jul 17, 2019·Antioxidants & Redox Signaling·Verena Nadin FritschHaike Antelmann
Mar 20, 2013·Molecular Microbiology·Changhan LeeChankyu Park
Feb 27, 2015·Biological Chemistry·Melanie Hillion, Haike Antelmann
Jun 8, 2021·Molecular Microbiology·Ankita J SachlaJohn D Helmann

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