The parasitic nematode Oesophagostomum dentatum synthesizes unusual glycosaminoglycan-like O-glycans

Glycobiology
Jorick VanbeselaereIain B H Wilson

Abstract

O-glycosylation is probably one of the most varied sets of post-translational modifications across all organisms, but amongst the most refractory to analyze. In animals, O-xylosylation of serine residues represents the first stage in the synthesis of glycosaminoglycans, whose repeat regions are generally analyzed as fragments resulting from enzymatic or chemical degradation, whereas their core regions can be isolated by β-elimination or endo-β-xylosidase digestion. In the present study, we show that hydrazinolysis can be employed for release of glycosaminoglycan-type oligosaccharides from nematodes prior to fluorescent labeling with 2-aminopyridine. While various [HexNAcHexA]nGal2Xyl oligosaccharides were isolated from the model organism Caenorhabditis elegans, more unusual glycosaminoglycan-type glycans were found to be present in the porcine parasite Oesophagostomum dentatum. In this case, as judged by MS/MS before and after hydrofluoric acid or β-galactosidase digestion, core sequences with extra galactose and phosphorylcholine residues were detected as [(±PC)HexNAcHexA]n(±PC)Galβ3-(±Galβ4)Galβ4Xyl. Thus, hydrazinolysis and fluorescent labeling can be combined to analyze unique forms of O-xylosylation, including new examples...Continue Reading

References

Apr 1, 1986·The Biochemical Journal·P N Shaklee, H E Conrad
Oct 20, 1999·FEBS Letters·S YamadaK Sugahara
Oct 11, 2011·Nature Chemical Biology·Mellisa LyRobert J Linhardt
Feb 22, 2012·International Journal for Parasitology·Katharina PaschingerIain B H Wilson
Apr 20, 2012·Molecular BioSystems·Lingyun LiRobert J Linhardt
Sep 17, 2013·Applied Microbiology and Biotechnology·Martin DragositsDubravko Rendic
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianzhong WenJack E Dixon
Mar 6, 2015·Glycobiology·Katharina Paschinger, Iain B H Wilson
May 24, 2015·Molecular & Cellular Proteomics : MCP·Shi YanKatharina Paschinger
Aug 27, 2015·Biomolecules·Kristian Prydz
Nov 7, 2015·Glycobiology·Ajit VarkiStuart Kornfeld
Oct 16, 2016·Methods in Molecular Biology·Alba HykollariIain B H Wilson
Oct 22, 2016·Biochimica Et Biophysica Acta. General Subjects·Carmen Jiménez-CastellsKatharina Paschinger
Nov 8, 2017·Journal of the American Chemical Society·Yanlei YuRobert J Linhardt
Nov 16, 2017·The Journal of Biological Chemistry·Fredrik NobornGöran Larson

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Citations

Jul 7, 2019·Glycoconjugate Journal·Katharina Paschinger, Iain B H Wilson
May 3, 2019·Parasitology·Katharina Paschinger, Iain B H Wilson
Mar 28, 2019·Frontiers in Molecular Biosciences·Katharina PaschingerIain B H Wilson
Apr 9, 2019·Open Biology·Feifei ZhuKeping Chen
May 7, 2021·Mass Spectrometry Reviews·Alba HykollariIain B H Wilson

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