PMID: 8614620Apr 1, 1996Paper

The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context

Nucleic Acids Research
S BiebingerC E Clayton

Abstract

African trypanosomes are extracellular protozoan parasites that are transmitted from one mammalian host to the next by tsetse flies. Bloodstream forms express variant surface glycoprotein (VSG); the tsetse fly (procyclic) forms express instead the procyclic acidic repetitive protein (PARP). PARP mRNA is abundant in procyclic forms and almost undetectable in blood-stream forms. Post-transcriptional mechanisms are mainly responsible for PARP mRNA regulation but results of nuclear run-on experiments suggested that transcription might also be regulated. We measured the activity of genomically-integrated PARP, VSG and rRNA promoters in permanently-transformed blood-stream and procyclic form trypanosomes, using reporter gene constructs that showed no post-transcriptional regulation. When the constructs were integrated in the rRNA non-transcribed spacer, the ribosomal RNA and VSG promoters were not developmentally regulated, but integration at the PARP locus reduced rRNA promoter activity in bloodstream forms. PARP promoter activity was 5-fold down-regulated in bloodstream forms when integrated at either site. Regulation was probably at the level of transcriptional initiation, but elongation through plasmid vector sequences was also r...Continue Reading

References

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Citations

Apr 1, 1996·Molecular and Biochemical Parasitology·C Clayton, H R Hotz
Nov 19, 2003·Microbes and Infection·David A CampbellNancy R Sturm
Nov 23, 2000·Molecular and Biochemical Parasitology·D J LaCountJ E Donelson
May 3, 2002·Molecular and Biochemical Parasitology·Maciej DrozdzChristine E Clayton
May 14, 2003·Molecular and Biochemical Parasitology·Daniëlle te VruchteGloria Rudenko
Feb 1, 1997·Molecular and Biochemical Parasitology·D Horn, G A Cross
Mar 1, 1997·Molecular and Biochemical Parasitology·S BiebingerC Clayton
May 9, 1998·Molecular and Biochemical Parasitology·P BorstG Rudenko
May 9, 1998·Molecular and Biochemical Parasitology·G A CrossM Navarro
May 9, 1998·Molecular and Biochemical Parasitology·L Vanhamme, E Pays
May 9, 1998·Molecular and Biochemical Parasitology·H R HotzC Clayton
Sep 7, 1999·Molecular and Biochemical Parasitology·L VanhammeE Pays
May 11, 1999·Parasitology Today·K ErsfeldK Gull
Jun 26, 2001·Trends in Parasitology·L VanhammeJ D Barry
Apr 3, 2002·The EMBO Journal·Maciej DrozdzKenneth Stuart
Apr 16, 2002·The EMBO Journal·Christine E Clayton
Jul 15, 2009·Nucleic Acids Research·Angela SchwedeChristine Clayton
Apr 10, 2003·Eukaryotic Cell·Gusti M ZeinerDavid A Campbell
Apr 12, 2005·Eukaryotic Cell·Nick DowneyDan S Ray
Apr 26, 2012·PloS One·Benard A OunaChristine Clayton
Aug 26, 1999·Parasitology Today·C E Clayton
Jun 1, 2001·Experimental Parasitology·L M WenG A Buck
Jun 7, 2006·Molecular Membrane Biology·Cédric YernauxPaul A M Michels
Feb 28, 2002·The Journal of Cell Biology·Nathan R HutchingsKent L Hill
Oct 14, 2008·Molecular and Biochemical Parasitology·Jared R HelmJohn E Donelson
Dec 22, 2004·Molecular and Biochemical Parasitology·Vincent P AlibuDavid Horn
Sep 9, 2010·Essays in Biochemistry·Gloria Rudenko
Jul 6, 2014·Molecular and Biochemical Parasitology·C E Clayton
May 14, 2016·Current Opinion in Microbiology·C E Clayton

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