Sep 1, 1976

The partial replacement of the serum growth factor requirement of SV3T3 cells by lactalbumin hydrolyzate

Journal of Cellular Physiology
D V Young

Abstract

Lactalbumin hydrolyzate (LH), a commercially prepared, enzymatic digest of a milk protein fraction, can partially replace the serum requirement of SV3T3 cells. In a low, but obligatory, background of calf serum (0.15% v/v), LH carses a large increase in the final cell density (5-10 x over the control) while modestly steimulating the actual growth rate. Lactalbumin hydrolyzate does not contain survival activity for SV3T3 cells and does not affect the growth of 3T3 cells. Since even prolonged exposure to pH 4 or 2 results in complete abolishment of the growth-rate stimulatory capability without affecting the capacity to increase the final cell density, it is possible that the LH growth activity may consist of two dissociable components. All of LH growth activity is water soluble, autoclavable, and resists proteolytic treatment. On Sephadex G15 growth activity appears as a single peak at the void volume but on G25 it is retained beyond the void volume as a broad, skewed peak. The relevant molecular weight range lies between 1,500 and 4,000. The putative low pH resistant material is strongly adsorbed to Dowex 1 x 2 and can be displaced from the column by a reduction in the pH. A comparison of the properties of the LH factors with t...Continue Reading

  • References23
  • Citations10

References

  • References23
  • Citations10

Citations

Mentioned in this Paper

Growth Substances
Cell Division Phases
Blood
Cell Survival
Lactalbumin
Hydrogen-Ion Concentration
Neoplastic Cell Transformation

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