The participation of proliferative keratinocytes in the preimmune response to sensitizing agents

The British Journal of Dermatology
M C LittleS Mac Neil

Abstract

The aims of this study were to investigate whether keratinocytes are capable of playing a direct preimmune role in the pathophysiology of allergic contact dermatitis (ACD) and to examine to what extent the degree of differentiation might influence this. We measured the ability of sensitizing agents to up-regulate intercellular adhesion molecule 1 (ICAM-1) expression in cultured normal human keratinocytes (NHK) and in the transformed human keratinocyte HaCaT cell line. In proliferative HaCaT cells, following a 24 h exposure, nickel compounds, para-phenylenediamine (pPD) and 1-chloro-2,4-dinitrobenzene produced a concentration-dependent up-regulation of ICAM-1 expression without reducing cell viability, while K2Cr2O7 led to ICAM-1 up-regulation at cytotoxic concentrations, and CrCl3 was without effect. In NHK, NiSO4 and pPD induced ICAM-1 expression to a significantly greater extent in proliferative cells than in differentiated cells, where involucrin expression was measured to assess the differentiation state. NiSO4- or pPD-pretreatment of proliferative HaCaT cells enhanced T-cell binding, which was abolished by neutralizing antibodies to ICAM-1 or CD18. Our investigations concerning the involvement of oxidative stress in the in...Continue Reading

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Citations

Mar 17, 2004·Toxicology and Applied Pharmacology·Monica LambertiMariateresa Giuliano
Feb 22, 2001·Free Radical Biology & Medicine·J FuchsM Podda
Sep 17, 2002·Antioxidants & Redox Signaling·Kevin J TroubaDori R Germolec
Jul 23, 1999·Contact Dermatitis·F SjögrenC Anderson
Jan 6, 2012·The Journal of Investigative Dermatology·Danuta Gutowska-Owsiak, Graham S Ogg
Mar 31, 2004·Scandinavian Journal of Immunology·G BanerjeeG Raman
Apr 19, 2002·Nutrition and Cancer·B S Mackie, L E Mackie
Aug 15, 2001·Acta Odontologica Scandinavica·M H Thornhill

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