The PAS Domain-Containing Protein HeuR Regulates Heme Uptake in Campylobacter jejuni

MBio
Jeremiah G JohnsonVictor J DiRita

Abstract

Campylobacter jejuni is a leading cause of bacterially derived gastroenteritis. A previous mutant screen demonstrated that the heme uptake system (Chu) is required for full colonization of the chicken gastrointestinal tract. Subsequent work identified a PAS domain-containing regulator, termed HeuR, as being required for chicken colonization. Here we confirm that both the heme uptake system and HeuR are required for full chicken gastrointestinal tract colonization, with the heuR mutant being particularly affected during competition with wild-type C. jejuni Transcriptomic analysis identified the chu genes-and those encoding other iron uptake systems-as regulatory targets of HeuR. Purified HeuR bound the chuZA promoter region in electrophoretic mobility shift assays. Consistent with a role for HeuR in chu expression, heuR mutants were unable to efficiently use heme as a source of iron under iron-limiting conditions, and mutants exhibited decreased levels of cell-associated iron by mass spectrometry. Finally, we demonstrate that an heuR mutant of C. jejuni is resistant to hydrogen peroxide and that this resistance correlates to elevated levels of catalase activity. These results indicate that HeuR directly and positively regulates ...Continue Reading

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Citations

Oct 29, 2018·Current Protocols in Microbiology·Brittni R KelleyJeremiah G Johnson
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Apr 16, 2019·Frontiers in Cellular and Infection Microbiology·Kaylie L RichardJeremiah G Johnson

Related Concepts

Metazoa
Bacterial Proteins
Catalase T
Heme
Perhydrol
Iron
Gene Expression Regulation, Bacterial
Campylobacter jejuni
MRNA Differential Display
Gastrointestinal Tract Structure

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