The patB gene of Dictyostelium discoideum encodes a P-type H(+)-ATPase isoform essential for growth and development under acidic conditions.

Microbiology
M Barrie CoukellAnne M Cameron

Abstract

During growth and early development of Dictyostelium discoideum, the amoebae exhibit transient pH changes in their cytosol (pHi) and external medium which correlate with the extrusion of H+ from the cell by a plasma membrane pump. Moreover, the changes in pHi have been postulated to influence early prestalk/prespore differentiation during development. To learn more about the role of H+ fluxes in Dictyostelium, we cloned and analysed cDNAs of the gene patB, which appears to encode a P-type H(+)-ATPase. The patB ORF encodes a protein (termed PAT2) of 1058 amino acids with a calculated molecular mass of 117,460 Da. When aligned with other P-type ion-transport ATPases, PAT2 showed the greatest amino acid sequence identity with plasma membrane H(+)-ATPases of plants and fungi and considerably lower identity with other monovalent cation pumps and with Ca2+ pumps. Northern and Western analyses revealed that patB is expressed at very low levels in cells growing at neutral pH, but it is up-regulated rapidly and dramatically when the cells are shifted to an acidic medium. Immunofluorescence analysis indicated that PAT2 resides on the plasma membrane. When patB was disrupted by homologous recombination, the cells grew and developed normal...Continue Reading

References

Feb 1, 1978·Biochimica Et Biophysica Acta·D MalchowG Gerisch
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J L Dynes, R A Firtel
Jul 1, 1988·Differentiation; Research in Biological Diversity·J D GrossR P von Strandmann
Nov 1, 1987·Molecular and Cellular Biology·J H McCuskerJ E Haber
Aug 17, 1987·FEBS Letters·R J AertsM M Van Lookeren Campagne
Oct 12, 1987·Nucleic Acids Research·C D Reymond
Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth
May 1, 1970·The Journal of Cell Biology·S M Cocucci, M Sussman
Dec 30, 1981·Biochimica Et Biophysica Acta·A Goffeau, C W Slayman
Oct 1, 1982·The Journal of Cell Biology·T J RinkT Pozzan
Dec 5, 1995·Biochemistry·S Lutsenko, J H Kaplan

❮ Previous
Next ❯

Citations

Sep 17, 2005·Journal of Bioenergetics and Biomembranes·Javier Barrero-GilBegoña Benito
Jan 30, 2002·Molecular and Biochemical Parasitology·Norma Marchesini, Roberto Docampo
Feb 19, 2002·Molecular and Biochemical Parasitology·Nicole Van Der Heyden, Roberto Docampo
Feb 29, 2000·Molecular and Biochemical Parasitology·N Van Der Heyden, R Docampo
Feb 29, 2000·Biochimica Et Biophysica Acta·A Rodríguez-Navarro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.