PMID: 7537356Feb 1, 1995Paper

The patch clamp technique

Neurosurgery
L K LiemK Tewari

Abstract

The introduction of the patch clamp technique less than two decades ago revolutionized the study of cellular physiology by providing a high-resolution method of observing the function of individual ionic channels in a variety of normal and pathological cell types. By the use of variations of the basic recording methodology, cellular function and regulation can be studied at a molecular level by observing currents through individual ionic channels. At a cellular level, processes such as signaling, secretion, and synaptic transmission can be examined. In addition, by combining the information from high-resolution electrophysiological recordings obtained by the patch clamp method with modern molecular biological techniques, further insight can be gained into the gene expression and protein structure of ionic channels. Given the ubiquity and importance of ionic channels, it is not surprising that their study has led to a new understanding of the mechanisms of certain disease processes and has given insight into treatments for these diseases. This review gives an historical perspective of the development of the patch clamp technique and an overview of the methodologies currently in use. Examples are shown to illustrate typical uses ...Continue Reading

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Citations

Apr 26, 2013·Chemical Reviews·Wiktor SzymańskiBen L Feringa
Nov 8, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·D Zhang
May 6, 2015·International Journal of Molecular Sciences·Jian ChenJunbo Wang
Feb 10, 2016·Differentiation; Research in Biological Diversity·Tim P KelderMonique R M Jongbloed
Mar 15, 2005·Biophysical Journal·Alan I Lee, James P Brody
Jul 13, 2006·Journal of Biomolecular Screening·Sui-Po ZhangEllen E Codd
Feb 1, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rachel Lees-GreenLeo K Cheng
Feb 12, 2021·Chembiochem : a European Journal of Chemical Biology·Johannes Broichhagen, Nicole Kilian

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