The path of electron transfer to nitrogenase in a phototrophic alpha-proteobacterium

Environmental Microbiology
Kathryn R FixenCaroline S Harwood

Abstract

The phototrophic alpha-proteobacterium, Rhodopseudomonas palustris, is a model for studies of regulatory and physiological parameters that control the activity of nitrogenase. This enzyme produces the energy-rich compound H2 , in addition to converting N2 gas to NH3 . Nitrogenase is an ATP-requiring enzyme that uses large amounts of reducing power, but the electron transfer pathway to nitrogenase in R. palustris was incompletely known. Here, we show that the ferredoxin, Fer1, is the primary but not sole electron carrier protein encoded by R. palustris that serves as an electron donor to nitrogenase. A flavodoxin, FldA, is also an important electron donor, especially under iron limitation. We present a model where the electron bifurcating complex, FixABCX, can reduce both ferredoxin and flavodoxin to transfer electrons to nitrogenase, and we present bioinformatic evidence that FixABCX and Fer1 form a conserved electron transfer pathway to nitrogenase in nitrogen-fixing proteobacteria. These results may be useful in the design of strategies to reroute electrons generated during metabolism of organic compounds to nitrogenase to achieve maximal activity.

References

Mar 3, 2019·Applied and Environmental Microbiology·Yanning Zheng, Caroline S Harwood
Jul 15, 2020·Annual Review of Microbiology·Caroline S Harwood

Citations

May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Nieva-GómezW J Brill
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May 22, 2010·Molecular Microbiology·Federico E Rey, Caroline S Harwood
Oct 5, 2010·Applied and Environmental Microbiology·Jean J HuangCaroline S Harwood
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
Apr 21, 2011·Geobiology·Eric S BoydJohn W Peters
Nov 9, 2011·Frontiers in Microbiology·Eric S BoydJohn W Peters
Jan 6, 2012·PLoS Computational Biology·Pan-Jun Kim, Nathan D Price
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May 27, 2015·International Journal of Molecular Sciences·Namita Khanna, Peter Lindblad
Mar 27, 2016·Current Opinion in Chemical Biology·John W PetersMichael W W Adams
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kathryn R FixenCaroline S Harwood
Aug 16, 2017·Journal of Bacteriology·Amaya M Garcia CostasJohn W Peters
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Feb 28, 2018·Journal of Bacteriology·Saroj PoudelEric S Boyd

Related Concepts

Metabolic Process, Cellular
Study
Biochemical Pathway
Cinnamoyl-CoA: Phenyllactate CoA-transferase Activity
Ferredoxin
Organic Chemicals
Electron Transport
Rana palustris
Ferredoxin Activity
Rosa palustris

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