PMID: 7547696Apr 1, 1995Paper

The pathogenic human monoclonal anti-DNA that induces experimental systemic lupus erythematosus in mice is encoded by a VH4 gene segment

International Immunology
A WaismanE Mozes

Abstract

Systemic lupus erythematosus (SLE) can be induced in mice by immunization with a human anti-DNA IgM mAb that was derived from a patient with cold agglutinin disease. The latter anti-DNA mAb expresses the common idiotype (Id) designated 16/6 Id. The original human hybridoma 16/6 that secreted an IgM antibody that bound ssDNA and carried the 16/6 Id had switched in culture to secrete an IgG molecule. Herein we show that the IgG 16/6 antibody contains the previously reported characteristics of the original IgM 16/6 mAb: it expresses the 16/6 Id and is capable of inducing experimental SLE in susceptible mouse strains. The identify of the IgG 16/6 anti-DNA mAb to the original IgM mAb was shown both by serological techniques and at the T cell level. The human IgG 16/6 mAb was found to be encoded by a germline gene from the human VH4 gene family, with high similarity to the germline gene VH4.21 that was previously shown to code for anti-DNA antibodies isolated from SLE patients. The VH4.21 germline gene was found to also code for most antibodies with cold agglutinin activity that were isolated from patients with cold agglutinin disease.

Citations

Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·A WaismanE Mozes
Jun 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Amir SharabiEdna Mozes
Feb 25, 2009·Expert Opinion on Biological Therapy·Noriko IikuniAntonio La Cava
Jul 28, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Amir SharabiEdna Mozes
Feb 14, 2015·The Journal of Allergy and Clinical Immunology·S Melkorka MaggadottirHakon Hakonarson
May 13, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Smadar GertelHoward Amital
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amir Sharabi, Edna Mozes
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Uri SelaEdna Mozes
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amir SharabiEdna Mozes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Hemolytic Anemia

Autoimmune hemolytic anemia (AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to an insufficient number of oxygen-carrying red blood cells in the circulation. Discover the latest research on AIHA here.

© 2021 Meta ULC. All rights reserved