PMID: 8599879Jan 1, 1996Paper

The pathogenic role of anti-phosphatidylserine antibodies: active immunization with the antibodies leads to the induction of antiphospholipid syndrome

Clinical Immunology and Immunopathology
O YodfatY Shoenfeld

Abstract

Primary antiphospholipid syndrome (pAPS) can be experimentally induced in mice by immunization with anti-cardiolipin (aCL) antibodies (Abs). Recently, we have pointed to the pathogenic role of antiphosphatidylserine (aPS) Abs by inducing an experimental model of pAPS in naive mice following passive transfer of human aPS to the tail vein of ICR mice. The aim of the present study was to induce experimental pAPS in mice following active immunization with aPS. Mice were immunized with IgG and IgM aPS, purified from two patients with pAPS. The sera of the mice were examined for the presence of different antiphospholipid Abs, and the beta 2 GPI dependency of aPS, using an enzyme-linked immunosorbent assay (ELISA). Inhibition ELISA studies, with silica beads coated with phospholipids, were used to detect cross-reactivity of aPS or aCL to other phospholipids. The mice were also tested for the presence of thrombocytopenia, prolonged activated partial thromboplastin time (APTT), and the percentage of fetal resorptions. The purified IgG aPS but not IgM aPS had a strong lupus anticoagulant activity. Both IgG and IgM aPS were beta 2 GPI dependent and did not bind PS in the absence of the glycoprotein. The mice immunized with IgG aPS, but no...Continue Reading

Citations

Jul 15, 1998·Arthritis and Rheumatism·R DesailloudJ C Piette
Jun 30, 2009·Clinical Reviews in Allergy & Immunology·Miri Blank, Yehuda Shoenfeld
Apr 12, 2003·Progress in Lipid Research·John A McIntyreW Page Faulk
Jul 28, 2001·European Journal of Clinical Investigation·Y ShoenfeldY Sherer
Sep 1, 2001·Clinical and Experimental Immunology·S KremmerH Grosse-Wilde
Jul 9, 2003·American Journal of Reproductive Immunology : AJRI·Shelly Tartakover MatalonYehuda Shoenfeld
Apr 14, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Der-Yuan ChenTsai-Ching Hsu
Oct 16, 2004·Lupus·M Blank, Y Shoenfeld
Feb 24, 2006·Blood·Gabriela Cesarman-MausKatherine A Hajjar

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Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

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