The pathogenic role of dendritic cells in non-infectious anterior uveitis

Experimental Eye Research
M O'RourkeM Canavan

Abstract

Anterior uveitis (AU) is characterised by infiltration of immune cells into the anterior chamber of the eye. Dendritic cells (DC) are professional antigen presenting cells that initiate and promote inflammation. This study aims to characterise DC in AU and to examine the effects of aqueous humor (AqH) on DC maturation and function. The frequency and phenotype of AU and healthy control (HC) circulating DC was examined. AU and HC AqH was immunostained and assessed by flow cytometry. The effect of AU and HC AqH on DC activation and maturation was examined and subsequent effects on CD4+ T cell proliferation assessed. AU peripheral blood demonstrated decreased circulating myeloid and plasmacytoid DC. Within AU AqH, three populations of CD45+ cells were significantly enriched compared to HC; DCs (CD11c+ HLA-DR+), neutrophils (CD15+ CD11c+) and T cells (CD4+ and CD8+). A significant increase in IFNγ, IL8 and IL6 was observed in the AU AqH, which was also significantly higher than that of paired serum. AU AqH induced expression of CD40 and CD80 on DC, which resulted in increased T cell proliferation and the production of GM-CSF, IFNγ and TNFα. DC are enriched at the site of inflammation in AU. Our data demonstrate an increase in inflam...Continue Reading

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Citations

Oct 10, 2018·The British Journal of Ophthalmology·Micheal O'RourkeConor C Murphy
Oct 26, 2018·Investigative Ophthalmology & Visual Science·Chan-Wei NienShun-Fa Yang
Oct 21, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Xinyue HuangPeizeng Yang
Apr 4, 2021·International Journal of Molecular Sciences·Dan-In JangSeung-Hoon Yang

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