PMID: 2499359May 30, 1989Paper

The pathway of electron transfer in NADH:Q oxidoreductase

Biochimica Et Biophysica Acta
R van Belzen, S P Albracht

Abstract

The pre-steady-state reduction by NADPH of NADH:Q oxidoreductase, as present in submitochondrial particles, has been further investigated with the rapid-mixing, rapid-freezing technique. It was found that trypsin treatment, that had previously been used to inactivate the transhydrogenase activity (Bakker, P.T.A. and Albracht, S.P.J. (1986) Biochim. Biophys. Acta 850, 413-422), considerably affected the stability at pH 6.2 of the NAD(P)H oxidation activity of submitochondrial particles. Use of the inhibitor butadione circumvented this problem, thus allowing a more careful investigation of the kinetics at pH 6.2. In the presence of the inhibitor rotenone it was found that 50% of the Fe-S clusters 3 and all of the Fe-S clusters 2 and 4 could be reduced by NADPH within 30 ms at pH 6.2. The remainder of the Fe-S clusters 3 and all of the Fe-S clusters 1 were reduced slowly (complete reduction only after more than 60 s). It was concluded that these latter Fe-S clusters play no role in the NADPH oxidation activity. In the absence of rotenone at pH 6.2 only 50% of the Fe-S clusters 2-4 could be reduced within 30 ms, while Fe-S cluster 1 was again not reduced. This difference was attributed to the fast reoxidation of part of the Fe-S cl...Continue Reading

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Citations

Aug 1, 1993·Journal of Bioenergetics and Biomembranes·M Finel
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·H Weiss, T Friedrich
Aug 30, 1990·Biochimica Et Biophysica Acta·A B Kotlyar, A D Vinogradov
Sep 1, 2007·Biochemistry·Vera G GrivennikovaAndrei D Vinogradov
Sep 1, 2009·Metallomics : Integrated Biometal Science·Wilfred R Hagen
Nov 1, 1992·European Journal of Biochemistry·R van BelzenS P Albracht
Sep 14, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Minnan LongSimon P J Albracht
Dec 1, 1991·Bioscience Reports·V P Skulachev
Jul 4, 1997·Biochimica Et Biophysica Acta·T V Zharova, A D Vinogradov
Jun 30, 1995·Biochimica Et Biophysica Acta·W M Anderson, D Trgovcich-Zacok
Jan 16, 1997·Biochimica Et Biophysica Acta·S P AlbrachtP de Jong
Feb 8, 1993·Biochimica Et Biophysica Acta·T Yagi
Jul 29, 1994·Biochimica Et Biophysica Acta·A M de JongS P Albracht
Sep 13, 1993·Biochimica Et Biophysica Acta·I E Hassinen, P T Vuokila
Jun 15, 1994·European Journal of Biochemistry·A M De Jong, S P Albracht
May 8, 1991·European Journal of Biochemistry·H WeissD Preis
Sep 17, 2003·The Journal of Biological Chemistry·Dirk FlemmingThorsten Friedrich
Sep 26, 2021·Structure·Johannes SchimpfThorsten Friedrich

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