PMID: 3757033Oct 10, 1986Paper

The pattern of actin expression in human fibroblast x mouse muscle heterokaryons suggests that human muscle regulatory factors are produced

Cell
E C HardemanH M Blau

Abstract

The expression of previously dormant human muscle genes encoding two major components of the contractile apparatus was activated in multinucleated heterokaryons formed by the fusion of mouse muscle cells and human fibroblasts. The accumulation of human and mouse alpha-cardiac and alpha-skeletal actin transcripts was compared by Northern blot, slot blot, and S1 nuclease assays. The pattern of human transcript accumulation in heterokaryons was quite distinct from that in the mouse muscle cells that induced it, and strikingly similar in time course and relative amounts to that in human primary muscle cultures. In addition, the usual decline in the level of mouse alpha-cardiac actin transcripts was not observed; instead, after fusion with human fibroblasts the levels increased. Our findings suggest that the activated human nuclei in heterokaryons produce their own muscle regulatory factors that alter the expression of mouse muscle genes and direct the expression of the human muscle phenotype.

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Citations

May 1, 1989·Somatic Cell and Molecular Genetics·G K PavlathH M Blau
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W L McKeehanG H Sato
Jun 27, 2009·Pathology Oncology Research : POR·Ferenc SiposBéla Molnár
May 1, 1991·Developmental Biology·L PajakD F Wieczorek
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Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T MiwaL Kedes
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I KaplanH M Blau
Apr 30, 2010·Rejuvenation Research·J Tkemaladze, K Chichinadze
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Jun 1, 1990·Differentiation; Research in Biological Diversity·R D CoxM E Buckingham
Jun 17, 2005·Annals of the New York Academy of Sciences·Diane S Krause
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Aug 2, 2003·Blood·Erica L HerzogDiane S Krause
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Dec 1, 1993·Current Opinion in Cell Biology·M H Baron

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