The pattern of Campylobacter contamination on broiler farms; external and internal sources

Journal of Applied Microbiology
T BattersbyD J Bolton

Abstract

The aim of this study was to apply the most sensitive molecular techniques in combination with culture-based methods to characterize broiler farms in terms of the timeline ('appearance' and 'pattern') of Campylobacter contamination prior to and post detection in the birds. Faecal and environmental samples were collected from three broiler farms (two flocks per farm). Real-time PCR was used to test for the presence of Campylobacter. Culture-based methods (enrichment and direct plating) were also applied and isolates were subject to a range of confirmatory tests before speciation (multiplex PCR). All flocks were colonized by Campylobacter before first thin and a similar pattern of Campylobacter contamination was observed; (day -1) a range of external and internal samples real-time PCR positive but culture negative; (day 0) chicks negative; (6-9 days pre-detection in the birds) internal samples (feeders, drinkers, barrier and/or bird weigh) culture positive and (post broiler infection) increasing concentrations of Campylobacter in internal samples but also on the tarmac apron and anteroom. It was concluded that; (i) vertical transmission did not occur; (ii) the environment was a potential source of Campylobacter; (iii) testing are...Continue Reading

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Citations

Apr 18, 2018·Transboundary and Emerging Diseases·I HanssonE O Engvall
Aug 19, 2020·Foodborne Pathogens and Disease·George NatsosKonstantinos C Koutoulis
Sep 11, 2018·Frontiers in Microbiology·Nompilo SibandaNicolae Corcionivoschi
Jun 19, 2019·Foodborne Pathogens and Disease·Simone de Fátima Rauber WürfelClarissa Silveira Luiz Vaz
Sep 3, 2020·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Marianne Chemaly
Jan 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Andrew D WalesJohn D Rodgers
Jan 9, 2021·Frontiers in Microbiology·Wenjun DengSteven C Ricke
Jul 31, 2021·Frontiers in Microbiology·Lucas HarrisonShaohua Zhao
Sep 23, 2021·Applied and Environmental Microbiology·F M CollesA L Smith

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