The pattern of histone H4 acetylation on the X chromosome during spermatogenesis of the desert locust Schistocerca gregaria

Genome Génome / Conseil National De Recherches Canada
K W Wolf, B M Turner

Abstract

We have used antibodies directed against histone H4 acetylated at lysine residue 5, 8, 12, or 16 and indirect immunofluorescence microscopy to probe chromosomes from spermatogonia and spermatocytes of the desert locust, Schistocerca gregaria. The autosomes showed bright overall fluorescence, indicative of high levels of H4 acetylation. In contrast, the X chromosome, which is facultatively heterochromatic during spermatogenesis of the locust, remained completely unstained in spermatogonia and secondary spermatocytes and showed only a small terminal fluorescent band in primary spermatocytes. This band probably corresponds to centromere associated constitutive heterochromatin. Thus, underacetylation is a cytogenetic marker for facultative heterochromatin, but not necessarily constitutive heterochromatin, during spermatogenesis of the locust. Scanning electron microscopy of chromosomes from prophase spermatogonia and prophase I spermatocytes revealed that underacetylation of histone H4 in the X chromosome was not accompanied by a chromatin organization visibly different from that of the autosomes. Transmission electron microscopy of mitotic spermatogonia showed that the X chromosome is separated from the autosomes in a small nuclea...Continue Reading

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Citations

Nov 20, 1998·Biological Chemistry·J BuzekB Vyskot
Jun 20, 1998·Mechanisms of Development·L Rastelli, M I Kuroda
Sep 7, 2011·Cytogenetic and Genome Research·C G Sotero-CaioM Guerra

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