The pattern of methacholine responsiveness in mice is dependent on antigen challenge dose

Respiratory Research
Graeme R ZoskyDebra J Turner

Abstract

Considerable variation exists in the protocols used to induce hyperresponsiveness in murine models of allergic sensitisation. We examined the effect of varying the number of antigen exposures at challenge on the development of methacholine responsiveness in systemically sensitised mice. BALB/c mice were sensitised with ovalbumin (OVA), challenged with 1, 3 or 6 OVA aerosols. Lung function was measured using low frequency forced oscillations and partitioned into components representing the airways (Raw) and lung parenchyma (tissue damping (G) and tissue elastance (H)). Responsiveness to inhaled methacholine (MCh), inflammatory cell profile and circulating IgE were assessed 24 and 48 hours after challenge. The threshold dose of MCh required to elicit a detectable response (sensitivity) and response to 30 mg x mL(-1) (maximal response) were determined for each compartment. Sensitivity; All three OVA protocols resulted in an increased sensitivity to MCh in Raw but not in G or H. These responses where present at 24 and 48 hrs, except 1 OVA aerosol in which changes had resolved by 48 hrs. Maximal response; 1 OVA aerosol increased maximal responses in Raw, G and H at 24 hrs, which was gone by 48 hrs. Three OVA aerosols increased respo...Continue Reading

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Citations

Mar 3, 2010·Immunology and Cell Biology·Matthew E WikstromPhilip A Stumbles
Jun 2, 2009·American Journal of Respiratory and Critical Care Medicine·Sam BayatWalid Habre
Mar 20, 2015·American Journal of Respiratory Cell and Molecular Biology·Khang M DuongSimon Phipps
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Jun 2, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Graeme R ZoskyPhilip A Stumbles
Feb 1, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Annette RobichaudThomas F Schuessler
Nov 26, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer T BurchellDebra J Turner

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Methods Mentioned

BETA
bronchoalveolar lavage
light microscopy
Assay

Software Mentioned

SigmaStat

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