The pattern recognition receptors dectin-2, mincle, and FcRγ impact the dynamics of phagocytosis of Candida, Saccharomyces, Malassezia, and Mucor species.

PloS One
Mohammed HaiderLars Peter Erwig

Abstract

Phagocytosis is a receptor-mediated process critical to innate immune clearance of pathogens. It proceeds in a regulated sequence of stages: (a) migration of phagocytes towards pathogens, (b) recognition of PAMPs and binding through PRRs, (c) engulfment and internalisation into phagosomes, (d) phagosome maturation, and (e) killing of pathogen or host cells. However, little is known about the role that individual receptors play in these discrete stages in the recognition of fungal cells. In a previous study, we found that dectin-2 deficiency impacted some but not all stages of macrophage-mediated phagocytosis of Candida glabrata. Because the C-type lectin receptor dectin-2 critically requires coupling to the FcRγ chain for signalling, we hypothesised that this coupling may be important for regulating phagocytosis of fungal cargo. We therefore examined how deficiency in FcRγ itself or two receptors to which it couples (dectin-2 and mincle) impacts phagocytosis of six fungal organisms representing three different fungal taxa. Our data show that deficiency in these proteins impairs murine bone marrow-derived macrophage migration, engulfment, and phagosome maturation, but not macrophage survival. Therefore, FcRγ engagement with sele...Continue Reading

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Citations

Mar 7, 2020·International Reviews of Immunology·Vijay Kumar
May 20, 2020·Chembiochem : a European Journal of Chemical Biology·Pablo ValverdeJesús Jiménez-Barbero
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Jul 21, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patrick K Nuro-GyinaJian Zhang

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Methods Mentioned

BETA
glycosylation
scraping

Software Mentioned

GraphPad Prism
Volocity
GraphPad

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