The PBDE metabolite 6-OH-BDE 47 affects melanin pigmentation and THRβ MRNA expression in the eye of zebrafish embryos

Endocrine Disruptors
Wu DongHeather M Stapleton

Abstract

Polybrominated diphenyl ethers and their hydroxyl-metabolites (OH-BDEs) are commonly detected contaminants in human serum in the US population. They are also considered to be endocrine disruptors, and are specifically known to affect thyroid hormone regulation. In this study, we investigated and compared the effects of a PBDE and its OH-BDE metabolite on developmental pathways regulated by thyroid hormones using zebrafish as a model. Exposure to 6-OHBDE 47 (10-100 nM), but not BDE 47 (1-50 μM), led to decreased melanin pigmentation and increased apoptosis in the retina of zebrafish embryos in a concentration-dependent manner in short-term exposures (4 - 30 hours). Six-OH-BDE 47 exposure also significantly decreased thyroid hormone receptor β (THRβ) mRNA expression, which was confirmed using both RT-PCR and in situ hybridization (whole mount and paraffin- section). Interestingly, exposure to the native thyroid hormone, triiodothyronine (T3) also led to similar responses: decreased THRβ mRNA expression, decreased melanin pigmentation and increased apoptosis, suggesting that 6-OH-BDE 47 may be acting as a T3 mimic. To further investigate short-term effects that may be regulated by THRβ, experiments using a morpholino gene knock do...Continue Reading

References

Mar 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C Charriaut-MarlangueY Ben-Ari
Feb 3, 1998·General and Comparative Endocrinology·K Yamano, S Miwa
Jan 11, 2002·Developmental Biology·L K Cole, L S Ross
May 23, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Keisuke Nakajima, Yoshio Yaoita
Dec 4, 2003·Thyroid : Official Journal of the American Thyroid Association·Iwan JonesDouglas Forrest
Dec 6, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wu DongTakeo Hiraga
Jul 24, 2004·Differentiation; Research in Biological Diversity·Stephanie M M Cossette, Thomas A Drysdale
Apr 8, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Timo HamersAbraham Brouwer
Jun 2, 2006·Molecular Pharmacology·Guilherme M SantosAbderrahim Lomri
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miriam ZeiniSonsoles Hortelano
Jul 7, 2007·Genes, Brain, and Behavior·M Rivas, J R Naranjo
Feb 6, 2008·Zebrafish·Keith C Cheng
Apr 30, 2008·Environmental Science & Technology·Antonius L van BoxtelJuliette Legler
Jun 5, 2008·Environmental Science & Technology·Heather M StapletonDonald G Patterson
Nov 4, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·David T SzaboLinda S Birnbaum
Nov 7, 2009·Reviews on Environmental Health·Susan D Shaw, Kurunthachalam Kannan
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stéphanie DecherfBarbara A Demeneix
Dec 17, 2010·The Journal of Clinical Endocrinology and Metabolism·Krystian JazdzewskiAlbert de la Chapelle
May 12, 2011·Environmental Toxicology and Chemistry·Crystal Y UsenkoErica D Bruce
Jul 28, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Margarita C Currás-Collazo
Aug 5, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lily NgDouglas Forrest
Dec 29, 2011·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·G S ArgumedoH J Olguín
Apr 20, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Weirong XingSubburaman Mohan
Jun 13, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke NakajimaYoshio Yaoita
Jul 31, 2012·General and Comparative Endocrinology·Alessandra SantilloGabriella Chieffi Baccari
Aug 14, 2012·Aquatic Toxicology·Chengyong HeChonggang Wang

❮ Previous
Next ❯

Citations

Apr 3, 2019·International Journal of Molecular Sciences·Lisa BaumannLucia Vergauwen
Jun 23, 2016·Environmental Toxicology and Chemistry·Laura J MacaulayHeather M Stapleton
Mar 5, 2016·Environmental Toxicology and Chemistry·Mingliang FangWu Dong
Aug 26, 2018·Journal of Cellular Physiology·Yupei WangHong Zhang
Nov 11, 2016·PloS One·Raúl GuillotJosé Miguel Cerdá-Reverter
Feb 7, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Erin M KollitzPatrick Lee Ferguson
Jun 26, 2020·Aquatic Toxicology·Lianguo Chen
Dec 15, 2015·Aquatic Toxicology·Melissa ChernickDavid E Hinton

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockdown
PCR
light microscopy
transfection

Software Mentioned

Applied
Biosystems Sequence Detection System
ImageJ
EclipseNet

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis