The pCS20 PCR assay for Ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the United States of America

The Onderstepoort Journal of Veterinary Research
S M MahanM J Burridge

Abstract

White-tailed deer are susceptible to heartwater (Ehrlichia [Cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the United States. It is vital, therefore, to validate a highly specific and sensitive detection method for E. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as Ehrlichia chaffeensis, Anaplasma (Ehrlichia) phagocytophilum (HGE agent) and Ehrlichia ewingii. Recently, a novel but as yet unnamed ehrlichial species, the white-tailed deer ehrlichia (WTDE), has been discovered in deer populations in the United States. Although the significance of WTDE as a pathogen is unknown at present, it can be distinguished from other Ehrlichia spp. based on 16S rRNA gene sequence analysis. In this study it was differentiated from E. ruminantium by the use of the pCS20 PCR assay which has high specificity and sensitivity for the detection of E. ruminantium. This assay did not amplify DNA from the WTDE DNA samples isolated from deer resident in Florida, Georgia and Missouri, but amplified the specific 279 bp fragment from E. ruminantium DNA. The specificity of the pCS20 PCR assay for E. rum...Continue Reading

Citations

Jun 7, 2005·Veterinary Parasitology·Mike TeglasJanet Foley
Jun 24, 2016·Parasites & Vectors·Abel BiguezotonChristine Chevillon
Oct 20, 2009·Veterinary Parasitology·Basil A Allsopp
Jun 9, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Olaoluwa Isaac AnifowoseEbenezer Babatunde Otesile

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