The PDZ domain of OutC and the N-terminal region of OutD determine the secretion specificity of the type II out pathway of Erwinia chrysanthemi

Journal of Molecular Biology
J BouleyVladimir E Shevchik

Abstract

The plant pathogens Erwinia chrysanthemi and Erwinia carotovora secrete multiple exoproteins by a type II pathway, the Out system. Secretion in Erwinia is species-specific: exoproteins of one species cannot be secreted by the other. We analysed the role of two components of the Out system, the bitopic inner membrane protein OutC and the secretin OutD, in the specific recognition of secreted proteins. We demonstrated that the PDZ domain of OutC determines its secretion specificity towards certain exoproteins. The secretin is the major determinant of specificity of the Out system: OutD of E. carotovora changes the secretion specificity of E. chrysanthemi and enables it to secrete heterologous exoproteins. Construction of chimeric OutD showed that the N-terminal region is the specificity domain of the secretin. Thus, both the PDZ domain of OutC and the N-terminal region of OutD are required for specific recognition of secreted proteins. Systematic analysis of the secretion of several exoproteins demonstrated that different exoproteins secreted by the Out machinery have different requirement for their presumed targeting signals on OutC and OutD. This strongly indicates that diverse exoproteins possess a variable number of targeting...Continue Reading

References

Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Y HeA Collmer
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Mar 1, 1993·Microbiological Reviews·A P Pugsley
Oct 24, 1996·Gene·D M DykxhoornT Linn
Feb 12, 1998·Molecular Microbiology·M J Pallen, C P Ponting
Oct 22, 1998·Molecular Microbiology·V E ShevchikN Hugouvieux-Cotte-Pattat
Jun 29, 1999·Journal of Bacteriology·O M PossotA P Pugsley
Jun 5, 2007·Molecular Microbiology·Séverine CollinAnthony P Pugsley

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Citations

Apr 3, 2012·Nature Reviews. Microbiology·Konstantin V KorotkovWim G J Hol
Sep 21, 2010·Nature Structural & Molecular Biology·Steve L ReichowTamir Gonen
Oct 10, 2009·The Journal of Biological Chemistry·Véronique ViarreRomé Voulhoux
Jan 19, 2012·The Journal of Biological Chemistry·Shuang GuRichard W Pickersgill
Jul 3, 2007·Molecular Plant-microbe Interactions : MPMI·Mathilde FagardDominique Expert
Mar 14, 2007·Journal of Bacteriology·Gérard P F MichelAlain Filloux
Nov 4, 2008·Journal of Bacteriology·Nienke BuddelmeijerAnthony P Pugsley
Mar 3, 2009·Journal of Bacteriology·Suzanne R LybargerMaria Sandkvist
Mar 12, 2013·Journal of Bacteriology·Susan HassanNicole Hugouvieux-Cotte-Pattat
Feb 20, 2003·Journal of Bacteriology·Minna Laatu, Guy Condemine
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·D Wade Abbott, Alisdair B Boraston
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Ian R HendersonDlawer Ala'Aldeen
Aug 21, 2010·Future Microbiology·Melissa AyersLori L Burrows
Jan 26, 2008·Environmental Microbiology·Flavia F EvansStaffan Kjelleberg
Sep 19, 2006·Journal of Molecular Biology·Konstantin V KorotkovWim G J Hol
Aug 8, 2014·Molecular Microbiology·Camille PineauVladimir E Shevchik
Jul 4, 2013·Journal of Structural Biology·Konstantin V KorotkovWim G J Hol
Jul 23, 2008·Molecular Microbiology·Yann Ferrandez, Guy Condemine
Feb 2, 2006·FEMS Microbiology Letters·Tanya L JohnsonMaria Sandkvist
Nov 8, 2011·Molecular Microbiology·S TammamP L Howell
Mar 27, 2015·Environmental Microbiology·Tiffany L LeightonLori L Burrows

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