The PDZ-GEF protein Dizzy regulates the establishment of adherens junctions required for ventral furrow formation in Drosophila

Journal of Cell Science
Philipp SpahnRolf Reuter

Abstract

The PDZ-GEF protein Dizzy (Dzy) and its downstream GTPase Rap1 have pleiotropic roles during development of the Drosophila embryo. Here, we show that maternally provided Dzy and Rap1 first function during ventral furrow formation (VFF) where they are critical to guarantee rapid apical cell constrictions. Contraction of the apical actomyosin filament system occurs independently of Dzy and Rap1, but loss of Dzy results in a delayed establishment of the apical adherens junction (AJ) belt, whereas in the absence of Rap1 only a fragmentary apical AJ belt is formed in the epithelium. The timely establishment of apical AJs appears to be essential for coupling actomyosin contractions to cell shape change and to assure completion of the ventral furrow. Immediately after VFF, the downregulation of Dzy and Rap1 is necessary to allow normal mesodermal development to continue after the epithelial-to-mesenchymal transition, as overexpression of Dzy or of constitutively active Rap1 compromises mesodermal migration and monolayer formation. We propose that Dzy and Rap1 are crucial factors regulating the dynamics of AJs during gastrulation.

References

Oct 1, 1986·The Journal of Cell Biology·D P Kiehart, R Feghali
Sep 20, 1988·Journal of Molecular Biology·N H Brown, F C Kafatos
Feb 2, 1999·The EMBO Journal·H AshaI K Hariharan
Dec 23, 1999·The Journal of Biological Chemistry·J de RooijJ L Bos
Jun 30, 2000·Biochemical and Biophysical Research Communications·A KawajiriK Kaibuchi
Aug 30, 2001·Trends in Genetics : TIG·D Bilder
Nov 9, 2001·Annual Review of Genetics·U TepassR Fehon
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·X MorinW Chia
Feb 16, 2002·Science·Andrea L Knox, Nicholas H Brown
Jan 29, 2003·The Journal of Cell Biology·Anne PacqueletPernille Rorth
Feb 13, 2003·Biochimica Et Biophysica Acta·H Bea KuiperijFried J T Zwartkruis
Jul 16, 2004·Molecular and Cellular Biology·Catherine HoganYasuyuki Fujita
Mar 2, 2005·Developmental Cell·Maria Leptin
Apr 28, 2005·Oncogene·Aurélien G DupuyJean de Gunzburg
Apr 29, 2005·The Journal of Biological Chemistry·Takashi HoshinoYoshimi Takai
Aug 27, 2005·Development·Rachel E Dawes-HoangEric F Wieschaus
Oct 7, 2005·Development·Donald T FoxMark Peifer
Sep 12, 2007·Molecular and Cellular Biology·Benjamin Boettner, Linda Van Aelst
Nov 26, 2008·Nature·Adam C MartinEric F Wieschaus
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simon de BecoSylvie Coscoy

❮ Previous
Next ❯

Citations

Sep 26, 2013·PloS One·Philipp Spahn, Rolf Reuter
Jul 28, 2015·Developmental Biology·Rupalatha MaddalaPonugoti V Rao
May 2, 2015·Developmental Biology·Allison K SpencerJeffrey H Thomas
Apr 30, 2013·Developmental Cell·Yu-Chiun WangEric F Wieschaus
Dec 3, 2013·Developmental Cell·Roberto MagliozziDaniele Guardavaccaro
Jan 20, 2017·Nature·Darren GilmourMaria Leptin
Apr 6, 2018·Journal of Cell Science·Anja Schmidt, Jörg Grosshans
May 16, 2014·Nature Reviews. Molecular Cell Biology·Masatoshi Takeichi
Aug 30, 2018·Molecular Biology of the Cell·Ketki SawantJocelyn A McDonald
Jul 3, 2020·Mechanisms of Development·Elham GheisariH-Arno J Müller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.