The pea END1 promoter drives anther-specific gene expression in different plant species

Planta
María D GómezLuis A Cañas

Abstract

END1 was isolated by an immunosubtractive approach intended to identify specific proteins present in the different pea (Pisum sativum L.) floral organs and the genes encoding them. Following this strategy we obtained a monoclonal antibody (mAbA1) that specifically recognized a 26-kDa protein (END1) only detected in anther tissues. Northern blot assays showed that END1 is expressed specifically in the anther. In situ hybridization and immunolocalization assays corroborated the specific expression of END1 in the epidermis, connective, endothecium and middle layer cells during the different stages of anther development. END1 is the first anther-specific gene isolated from pea. The absence of a practicable pea transformation method together with the fact that no END1 homologue gene exists in Arabidopsis prevented us from carrying out END1 functional studies. However, we designed functional studies with the END1 promoter in different dicot species, as the specific spatial and temporal expression pattern of END1 suggested, among other things, the possibility of using its promoter region for biotechnological applications. Using different constructs to drive the uidA (beta-glucuronidase) gene controlled by the 2.7-kb isolated promoter ...Continue Reading

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Citations

Apr 29, 2006·Natural Product Research·Thomas J SchmidtGünter Willuhn
Sep 1, 2012·BMC Plant Biology·Begoña García-SogoLuis Antonio Cañas
Jul 11, 2013·Plant Reproduction·María Dolores GómezLuis A Cañas
Apr 16, 2013·Plant Biotechnology Journal·Mónica MedinaConcepción Gómez-Mena
Feb 17, 2006·Current Opinion in Plant Biology·Claire DomoneyChristophe Salon
Sep 5, 2012·Biochemical and Biophysical Research Communications·Tirthartha ChattopadhyayMrinal K Maiti
Feb 14, 2020·Plant Cell Reports·M Valentina BrionesMatthias Fladung
Jan 1, 2014·Horticulture Research·Manjul DuttJude W Grosser
Jul 12, 2019·Frontiers in Plant Science·Edelín RoqueLuis A Cañas
Aug 28, 2020·Journal of Experimental Botany·Maria Dolores GomezMiguel A Perez-Amador
Dec 1, 2020·Plant Physiology and Biochemistry : PPB·Gabriel H J Robinson, Claire Domoney
Nov 16, 2021·Frontiers in Plant Science·Rim HamzaLuis A Cañas
Nov 26, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cecilia Martínez-MartínezAntonio J Monforte

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