The PEA3 protein of the Ets oncogene family is a putative transcriptional modulator of the mouse epididymis-specific glutathione peroxidase gene gpx5

Molecular Reproduction and Development
J R DrevetJ P Dufaure

Abstract

This report presents data that suggest that the tissue-restricted polyoma enhancer activator protein (PEA3) of the Ets oncogene family of DNA-binding proteins is a putative modulator of the epididymis-specific glutathione peroxidase 5 gene gpx5. Northern and polymerase chain reactions on reverse-transcribed epididymal RNAs were used to show that the PEA3 factor is spatially and temporally expressed within the mouse epididymis in a manner consistent with gpx5 characteristics of expression. Then, using contransfection experiments carried out in heterologous tissue-culture cells with various deletions of the gpx5 promoter driving a CAT reporter gene, we have shown that the transcriptional activity of the gpx5 promoter is modulated by the presence of the PEA3 protein. Subsequently, we have shown using gel-shift assays that DNA sequences located within the 5' flanking region of the gpx5 gene have the ability to bind specifically to the PEA3 protein. Finally, using Northern assays we present data that suggest that PEA3 mRNA accumulation in the mouse caput epididymidis is controlled by androgens and testicular factors. Altogether, these results strongly suggest that the PEA3 factor might participate in the transcriptional control of t...Continue Reading

References

Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Jun 1, 1991·Journal of Biochemistry·S YoshimuraT Moriuchi
Jan 1, 1989·Free Radical Biology & Medicine·I A ClarkW B Cowden
Mar 1, 1989·Journal of Reproduction and Fertility·N B GhyselinckJ P Dufaure
Feb 15, 1989·Analytical Biochemistry·D B Schowalter, S S Sommer
Jan 1, 1989·Free Radical Biology & Medicine·N J Schisler, S M Singh
Dec 1, 1988·Archives of Biochemistry and Biophysics·D E Ong, F Chytil
Oct 20, 1988·Nature·M Ptashne
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Feb 1, 1983·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·K AbeT Ito
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L ManleyM L Gefter
Aug 1, 1980·Nutrition Reviews·R A Sunde, W G Hoekstra
Jun 25, 1993·Biochimica Et Biophysica Acta·S HimenoN Imura
Feb 1, 1993·Biology of Reproduction·R S PappasD E Ong
May 1, 1993·Molecular Reproduction and Development·M A WinerD J Wolgemuth
Jan 1, 1995·Reproduction, Fertility, and Development·B T HintonJ C Labus

❮ Previous
Next ❯

Citations

Apr 28, 2004·Molecular and Cellular Endocrinology·Kichiya SuzukiMarie-Claire Orgebin-Crist
Oct 17, 2003·Biology of Reproduction·Nelson Hsia, Gail A Cornwall
Nov 8, 2001·DNA Sequence : the Journal of DNA Sequencing and Mapping·A A PedersenB K Michelsen
Nov 3, 2006·Biology of Reproduction·Takayuki HoshiiKen-ichi Yamamura
Nov 8, 2014·Biology of Reproduction·Julie Dufresne, Daniel G Cyr
Apr 28, 2004·Molecular and Cellular Endocrinology·Christiane KirchhoffMarie-Claire Orgebin-Crist

❮ Previous
Next ❯

Related Concepts

Related Feeds

BK Virus Infection

BK virus infection is a significant complication of modern immunosuppression used in kidney transplantation. Discover the latest research on BK virus infection here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.