The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

MSphere
Matthias WehrmannJanosch Klebensberger

Abstract

In Pseudomonas putida KT2440, two pyrroloquinoline quinone-dependent ethanol dehydrogenases (PQQ-EDHs) are responsible for the periplasmic oxidation of a broad variety of volatile organic compounds (VOCs). Depending on the availability of rare earth elements (REEs) of the lanthanide series (Ln3+), we have recently reported that the transcription of the genes encoding the Ca2+-utilizing enzyme PedE and the Ln3+-utilizing enzyme PedH are inversely regulated. With adaptive evolution experiments, site-specific mutations, transcriptional reporter fusions, and complementation approaches, we now demonstrate that the PedS2/PedR2 (PP_2671/PP_2672) two-component system (TCS) plays a central role in the observed REE-mediated switch of PQQ-EDHs in P. putida We provide evidence that in the absence of lanthanum (La3+), the sensor histidine kinase PedS2 phosphorylates its cognate LuxR-type response regulator PedR2, which in turn not only activates pedE gene transcription but is also involved in repression of pedH Our data further suggest that the presence of La3+ lowers kinase activity of PedS2, either by the direct binding of the metal ions to the periplasmic region of PedS2 or by an uncharacterized indirect interaction, leading to reduced l...Continue Reading

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Jan 18, 2019·Molecular Microbiology·Andrea M OchsnerJulia A Vorholt
Jan 24, 2019·Molecular Microbiology·Ludmila Chistoserdova
Apr 25, 2019·Chembiochem : a European Journal of Chemical Biology·Emily R FeatherstonJoseph A Cotruvo
Sep 11, 2019·Environmental Microbiology Reports·Sarah DethlefsenBurkhard Tümmler
Aug 23, 2020·Applied and Environmental Microbiology·Mitchell G ThompsonJay D Keasling
Sep 27, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Emily R Featherston, Joseph A Cotruvo
Sep 14, 2021·Frontiers in Bioengineering and Biotechnology·Yidong LiuCuiqing Ma

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Methods Mentioned

BETA
PCR

Software Mentioned

Clustal Omega
Simple Modular Architecture Research Tool

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