The Pepsin Hydrolysate of Bovine Lactoferrin Causes a Collapse of the Membrane Potential in Escherichia coli O157:H7

Probiotics and Antimicrobial Proteins
C A MurdockKarl R Matthews

Abstract

In the present study, the ability of bovine lactoferrin hydrolysate (LfH) to disrupt the cytoplasmic membrane of Escherichia coli O157:H7 was investigated. Lactoferrin and LfH antimicrobial activities were compared against E. coli O157:H7 and E. coli O157:H7 spheroplasts. The effect of LfH on the cytoplasmic membrane of E. coli O157:H7 cells was determined by evaluating potassium efflux (K(+)), dissipation of ATP and membrane potential (ΔΨ). LfH produced a rapid efflux of potassium ions, a decrease in intracellular levels of ATP coupled with a substantial increase in extracellular ATP levels and a complete dissipation of the ΔΨ. The results suggest that LfH causes a collapse of the membrane integrity by pore formation in the inner membrane, leading to the death of the cell. Moreover, the mechanism of action of LfH on E. coli O157:H7 appears to involve an interference with the inner membrane integrity based on experiments using E. coli O157:H7 spheroplasts.

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Citations

May 6, 2016·Probiotics and Antimicrobial Proteins·Aabha GuptaMichael L Chikindas
Jun 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natascia BruniFranco Dosio
Feb 15, 2018·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Evelien KieckensDaisy Vanrompay
Dec 21, 2014·Applied and Environmental Microbiology·Santosh Kumar TiwariMichael L Chikindas
Mar 1, 2012·Probiotics and Antimicrobial Proteins·Yen Nee TanMohd Khan Ayob
Jun 1, 2013·Probiotics and Antimicrobial Proteins·Wen-Hsuan WuKarl R Matthews
Apr 10, 2019·Foodborne Pathogens and Disease·Qiuxia FanXiaodong Xia

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