The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides.

Applied and Environmental Microbiology
D SchwartzW Wohlleben

Abstract

By complementation of a previously described non-phosphinothricin tripeptide (PTT)-producing mutant, NTG1, which is blocked in nonribosomal synthesis of the peptide, a DNA fragment including the putative peptide synthetase gene phsA was isolated (W. Wohlleben, R. Alijah, J. Dorendorf, D. Hillemann, B. Nussbaumer, and S. Pelzer, Gene 115:127-132, 1992). Sequence analysis of phsA revealed that it encodes a protein of 622 amino acids with regions which are highly similar to core motifs characteristic for peptide synthetases. PhsA represents one functional domain of a peptide synthetase which is necessary for activation and condensation of one amino acid, probably N-acetyl-demethyl-phosphinothricin. With regard to the arrangement of the flanking genes, phsA is the first peptide synthetase gene which is not in the direct neighborhood of additional peptide synthetase genes involved in the formation of peptide antibiotics. Gene disruption mutants with internal fragments of phsA subcloned in temperature-sensitive pGM vectors were generated. Integration occurred either into the chromosomal copy of phsA or into a gene outside the known phsA locus, resulting in two classes of non-PTT-producing mutants. In cofeeding experiments the former ...Continue Reading

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Citations

Dec 3, 2004·Applied and Environmental Microbiology·Dirk SchwartzWolfgang Wohlleben
Oct 28, 2005·Antimicrobial Agents and Chemotherapy·Dirk SchwartzWolfgang Wohlleben
Nov 3, 2012·Environmental Microbiology·Ewelina MichtaYvonne Mast
Oct 12, 2000·FEMS Microbiology Letters·M C Moffitt, B A Neilan
Jun 6, 2009·Annual Review of Biochemistry·William W Metcalf, Wilfred A van der Donk
Jul 17, 1998·Current Opinion in Chemical Biology·H D Mootz, M A Marahiel
Oct 28, 2016·Chemical Reviews·Geoff P Horsman, David L Zechel
Jan 6, 2018·ACS Chemical Biology·Emily C UlrichWilfred A van der Donk
Feb 20, 2002·Chemical Reviews·Hans von DöhrenRainer Zocher

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