PMID: 590269Nov 15, 1977

The peptidoglycan crosslinking enzyme system in Streptomyces strains R61, K15 and rimosus. Kinetic coefficients involved in the interactions of the membrane-bound transpeptidase with peptide substrates and beta-lactam antibiotics

European Journal of Biochemistry
J DusartJ M Ghuysen

Abstract

The transpeptidation reaction performed by the membranes of Streptomyces strain R61 fits the general rate equation for an enzyme-catalysed bimolecular reaction. The same membranes (E) interact with beta-lactams (I) to form inactive penicillin-enzyme-membrane complexes (EI) of rather high stability, which subsequently break down (E + I leads to EI leads to E + degradation products). The enzyme is regenerated and the antibiotic is released in the form of an inactive metabolite. With benzylpenicillin, the degradation product is benzylpenicilloic acid. The reaction is heat-labile. The first step of the reaction (E + I leads to EI) is characterized by a second-order rate constant (kformation in M-1 s-1) and the second step (EI leads to E + degradation products) by a first-order rate constant (kbreakdown in s-1). The effects in vitro of various beta-lactams on the membrane-bound transpeptidase, as expressed by the relevant kformation and kbreakdown values, parallel the effects in vivo of the same antibiotics as expressed by their ability to prevent the germination and growth of conidiospores. The kinetic parameters of the transpeptidase that was solubilized with N-cetyl-N,N,N-trimethylammonium bromide with respect to its interaction ...Continue Reading

Citations

Sep 7, 2002·Journal of Molecular Biology·Michael A McDonoughJudith A Kelly
Mar 1, 1979·European Journal of Biochemistry·D Mirelman, Y Nuchamowitz
Jan 1, 1985·Critical Reviews in Microbiology·J M Frère, B Joris
Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alexander J F EganWaldemar Vollmer
Feb 28, 2020·Clinical Microbiology Reviews·Karen Bush, Patricia A Bradford
Apr 29, 1998·Journal of Bacteriology·J Hao, K E Kendrick

Related Concepts

Muramoylpentapeptide Carboxypeptidase
Carboxypeptidase
Plasma Membrane
Van-Pen-G
Penicillin
Pseudomurein
Streptomyces
Substrate Specificity

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