The peptidyl-prolyl isomerase domain of the CyP-40 cyclophilin homolog Cpr7 is not required to support growth or glucocorticoid receptor activity in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
A A DuinaR F Gaber

Abstract

CyP-40 cyclophilins are found in association with molecular chaperone Hsp90.steroid receptor complexes. The amino-terminal portion of these cyclophilins harbors the characteristic peptidyl-prolyl isomerase (PPIase) domain, whereas three copies of the tetratricopeptide (TPR) motif, a structure shown to be involved in protein-protein interactions, and a putative calmodulin-binding domain are located in the carboxyl-terminal half of the protein. The TPR domains mediate binding to Hsp90, but a requirement for the PPIase domain has not been established. To address this, we have investigated the effects of mutations that alter the PPIase domain of the Saccharomyces cerevisiae CyP-40 homolog, Cpr7. Because Cpr7 is required for rapid growth and full Hsp90 activity, a functional assessment of the PPIase domain could be performed in vivo. A mutation in the catalytic domain altering a conserved site predicted to be essential for isomerase activity did not compromise Cpr7 function. Furthermore, deletion of the entire PPIase domain did not significantly affect growth or Hsp90-mediated steroid receptor activity. These results indicate that the TPR-containing carboxyl terminus of Cpr7 is sufficient for fundamental Cpr7-dependent activity.

References

Jul 9, 1992·Nature·H WiechU Jakob
Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·C RadanyiE E Baulieu
Sep 29, 1995·The Journal of Biological Chemistry·P A FerreiraW L Pak
Jul 1, 1995·Trends in Biochemical Sciences·J R LambP Hieter
Apr 1, 1995·The Biochemical Journal·K Hoffmann, R E Handschumacher
Apr 6, 1995·Biochemical and Biophysical Research Communications·T RatajczakR F Minchin
Jun 2, 1995·Science·S P BohenK R Yamamoto
Jul 1, 1995·Molecular and Cellular Biology·D F Nathan, S Lindquist
Dec 30, 1994·Cell·S L Rutherford, C S Zuker
Sep 15, 1993·European Journal of Biochemistry·A Galat
Dec 1, 1995·Molecular and Cellular Biology·D F SmithR A Rimerman
Feb 2, 1996·The Journal of Biological Chemistry·M YoneharaI Yahara
Jul 1, 1996·Physiological Reviews·A R Marks
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C SchneiderF U Hartl
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·D F NathanS Lindquist
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·K DolinskiJ Heitman

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Citations

Feb 19, 2000·Current Opinion in Structural Biology·L H Pearl, C Prodromou
Sep 13, 2011·The Journal of Biological Chemistry·Keith W Earley, R Scott Poethig
Mar 11, 2000·Microbiology and Immunology·R KanoA Hasegawa
Jun 8, 2006·Annual Review of Biochemistry·Laurence H Pearl, Chrisostomos Prodromou
Nov 28, 2013·Scientifica·Stuart K Calderwood
Aug 19, 2008·FEBS Letters·Tony C LuuWilliam K Chan
Nov 3, 2011·The EMBO Journal·Taichiro IkiMasayuki Ishikawa
Feb 3, 2015·Biochimica Et Biophysica Acta·Fiamma MantovaniGiannino Del Sal
Nov 9, 2014·Eukaryotic Cell·Victoria R TengeJill L Johnson
Dec 6, 2017·International Journal of Molecular Sciences·Gabriel R FriesTheo Rein
Sep 28, 2002·The Korean Journal of Parasitology·Hak-Sun YuDong-Il Chung
Dec 5, 1998·The Journal of Biological Chemistry·L A CarverC A Bradfield
Jun 6, 2003·The Journal of Biological Chemistry·Marija TesicRichard F Gaber
Feb 13, 2019·Cold Spring Harbor Perspectives in Biology·Maximilian M Biebl, Johannes Buchner
Sep 5, 2018·The Journal of Physical Chemistry. B·Katarzyna M TychMatthias Rief

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