The performance of an in-house loop-mediated isothermal amplification for the rapid detection of Mycobacterium tuberculosis in sputum samples in comparison with Xpert MTB/RIF, microscopy and culture

Revista Do Instituto De Medicina Tropical De São Paulo
Benjawan PhetsuksiriYasuhiko Suzuki

Abstract

Simple, low-cost and effective diagnostic tests for tuberculosis (TB) are needed especially in TB-high burden settings. The present study evaluated the performance of an in-house loop-mediated isothermal amplification (LAMP) for diagnosing TB by comparing it to Xpert MTB/RIF, microscopy and culture. In Thailand, a total of 204 excess sputum samples volume after the processing of cultures were used for Mycobacterium tuberculosis (MTB) detection by Xpert MTB/RIF and LAMP. Based on culture results as the gold standard, the overall sensitivity of LAMP and Xpert MTB/RIF were 82.1% (126/153; 95% confidential interval [CI]: 75.4-88.98%) and 86.9 % (133/153; 95% CI: 80.5-90.8%) respectively, and the specificity of both tests was 100% (51/51; 95% CI: 93.0-100.0%). In comparison with Xpert MTB/RIF, the sensitivity and specificity of LAMP were 94.7% (126/133; 95% CI: 89.5-97.9%), and 100.0% (73/73; 95% CI: 94.9-100.0%), respectively. The average threshold cycle (Ct) of Xpert MTB/RIF detection for positive and negative LAMP results was statistically different, of 18.4 and 27.0, respectively (p < 0.05). In comparison with the acid-fast staining technique, and analyzing LAMP and Xpert MTB/RIF in smear-negative/culture-positive specimens, the...Continue Reading

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Jul 21, 2012·Japanese Journal of Infectious Diseases·Janisara RudeeaneksinBenjawan Phetsuksiri
Sep 16, 2017·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R YadavS Sethi
Dec 17, 2017·Diagnostic Microbiology and Infectious Disease·Van Anh Thi NguyenNhung Viet Nguyen
Feb 8, 2018·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Thu Hang PhamMark D Perkins
Jun 27, 2017·Journal of Infection in Developing Countries·Charity HabeenzuTakashi Matsuba

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Methods Mentioned

BETA
nucleic acid amplification
electrophoresis

Software Mentioned

GeneXpert
SAS

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