The performance of hybrid monolithic silica capillary columns prepared by changing feed ratios of tetramethoxysilane and methyltrimethoxysilane

Journal of Chromatography. a
Takeshi HaraNobuo Tanaka

Abstract

The effect of a feed ratio of methyltrimethoxysilane (MTMS) to tetramethoxysilane (TMOS) was studied to improve the performance of a hybrid monolithic silica capillary column with 100-mum i.d. in HPLC in a range MTMS/TMOS (v/v)=10/90-25/75. The domain size was also varied by adjusting the amount of PEG to control permeability (K=2.8x10(-14)-6.9x10(-14)m(2)). Evaluation of the performance for those capillary columns following octadecylsilylation proved an increase in retention factor (k) and a decrease in steric selectivity alpha(triphenylene/ortho-terphenyl) with the increase in MTMS content in the feed. The effect of the feed ratio was also observed in porosity and hydrophobic property of the C18 stationary phase from the results of size exclusion chromatography (SEC) and reversed phase characterization. The monolithic silica capillary columns prepared under new preparation conditions were able to produce a plate height of 4.6-6.0microm for hexylbenzene in a mobile phase acetonitrile/water=80/20 at a linear velocity of 2mm/s. Consequently, it was possible to prepare hybrid monolithic silica capillary columns with higher performance than those reported previously while maintaining the retention factors in a similar range by red...Continue Reading

References

Sep 19, 2002·Journal of Chromatography. a·J J KirklandR D Ricker
Jun 27, 2006·Journal of Chromatography. a·A-M Siouffi
Jul 21, 2007·Journal of Chromatography. a·Georges Guiochon

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Citations

Dec 5, 2012·Analytical and Bioanalytical Chemistry·Yu LiangYukui Zhang
Nov 23, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoyan JiaoTiesheng Shi
Oct 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Racha El-DebsClaire Demesmay
Apr 18, 2016·Journal of Chromatography. a·Takeshi HaraGert Desmet
Sep 28, 2016·Journal of Separation Science·Zuzana Zajickova
May 27, 2017·Physical Chemistry Chemical Physics : PCCP·Rafael MeinuschBernd M Smarsly
Jan 20, 2019·Journal of Separation Science·Zuzana Zajickova, Ivan Špánik
Dec 21, 2013·The Analyst·David A CollinsBrett Paull
Jun 8, 2011·Journal of Separation Science·Dzmitry HlushkouUlrich Tallarek
Oct 23, 2014·Chemistry : a European Journal·Ronald GöbelAndreas Taubert
Jan 1, 2016·Journal of Separation Science·El-Debs RachaClaire Demesmay
Apr 15, 2011·Journal of Separation Science·Ashraf Ghanem, Tohru Ikegami
Jul 10, 2017·Journal of Chromatography. a·Shunta FutagamiWim De Malsche
Aug 6, 2010·Analytical Chemistry·Dzmitry HlushkouUlrich Tallarek
Aug 7, 2010·Analytical Chemistry·Frederik DetobelGert Desmet
Aug 15, 2019·Analytical Chemistry·Martyna BacaWim De Malsche
Nov 20, 2012·Analytical Chemistry·Gert Desmet, Sebastiaan Eeltink
Apr 19, 2011·Journal of Chromatography. a·Pengfei ZhangLi Jia

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