The performance of tofu-whey as a liquid medium in the propagation of mycobacterium tuberculosis strain H37Rv

International Journal of Mycobacteriology
Frederick R Masangkay

Abstract

To investigate the performance of "tofu-whey liquid medium" for the propagation of Mycobacterium tuberculosis (MTB) strain H37Rv. Two hundred micro liters (200μl) of 1 McFarland standard (1mg/ml-bacillary suspension) were inoculated into different batches of tofu-whey liquid medium. Each series contained three trials of test (tofu-whey liquid medium) and control media (Middlebrook 7H9 medium). Turbidity was measured within three weeks of inoculation using a nephelometer. The combinations of various tofu-whey liquid culture media were as follows; T1 (tofu-whey+ADC+glycerol+Potassium sulfate+Magnesium citrate+Sodium glutamate); T2 (tofu-whey+ADC+glycerol+Potassium sulfate+Magnesium citrate); T3 (tofu-whey+ADC+glycerol+Potassium sulfate); T4 (tofu-whey+ADC+glycerol); T5 (tofu-whey+ADC); and T6 (tofu-whey only). In all test and control liquid culture media, the multiplication of M. tuberculosis was documented under light and fluorescence microscopy. Of various tofu-whey medium used, T1 demonstrated the most potential for MTB propagation. The increased turbidity reading represented by the value in "unit drop of % transmittance" was higher (25 scores) in the T1 tofu-whey medium, compared with the T6 tofu-whey medium (8 scores). The o...Continue Reading

References

Sep 1, 1973·The British Journal of Nutrition·P LunvenA Fratoni
May 5, 2000·Diagnostic Microbiology and Infectious Disease·M V KanchanaT Martin
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M D Pinheiro, M M Ribeiro
May 1, 2002·Journal of Clinical Microbiology·Gaby E PfyfferSabine Rüsch-Gerdes
Oct 29, 2003·International Journal of Food Microbiology·Nguyen Thi LêJacques Goulet
Jul 13, 2006·Natural Product Research·Po S Poon Ng, Ajoy K Banerjee
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Erik C Hett, Eric J Rubin
Dec 16, 2009·International Journal of Dermatology·Archana Singal, Puneet Aggarwal
Sep 9, 2009·Indian Journal of Medical Microbiology·R K SharmaS S Malvankar
May 1, 1946·The Journal of Experimental Medicine·R J DUBOS, B D DAVIS

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