The periplasmic disordered domain of RodZ promotes its self-interaction in Escherichia coli

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Ryosuke IkebeDaisuke Shiomi

Abstract

Rod shape of bacterial cells such as Escherichia coli is mainly regulated by a supramolecular complex called elongasome including MreB actin. Deletion of the mreB gene in rod-shaped bacterium E. coli results in round-shaped cells. RodZ was isolated as a determinant of rod shape in E. coli, Caulobacter crescentus and Bacillus subtilis and it has been shown to be an interaction partner and a regulator of assembly of MreB through its cytoplasmic domain. As opposed to functions of the N-terminal cytoplasmic domain of RodZ, functions of the C-terminal periplasmic domain including a disordered region are still unclear. To understand it, we adopted an in vivo photo-cross-linking assay to analyze interaction partners to identify proteins which interact with RodZ via its periplasmic domain, finding that the RodZ self-interacts in the periplasmic disordered domain. Self-interaction of RodZ was affected by MreB actin. Deletion of this region resulted in aberrant cell shape. Our results suggest that MreB binding to the cytoplasmic domain of RodZ causes structural changes in the disordered periplasmic domain of RodZ. We also found that the disordered domain of RodZ contributes to fine-tune rod shape in E. coli.

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Citations

Feb 12, 2019·Molecular Microbiology·Yusuke YoshiiDaisuke Shiomi
Jan 10, 2020·AIMS Microbiology·Risa Ago, Daisuke Shiomi
Jul 20, 2018·Nature Communications·Benjamin P BrattonRandy M Morgenstein
Dec 29, 2020·PLoS Genetics·Xiaolong LiuTanneke den Blaauwen
Jun 30, 2021·Annual Review of Cell and Developmental Biology·Ethan C Garner

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