PMID: 5901498Jan 1, 1966

The permeability of rat transitional epithelium. Kertinization and the barrier to water

The Journal of Cell Biology
R M Hicks

Abstract

Permeability barriers must exist in transitional epithelium to prevent the free flow of water from underlying blood capillaries through the epithelium into the hypertonic urine, and such a barrier has now been demonstrated in isolated bladders. This barrier is passive in function and can be destroyed by damaging the luminal surface of the transitional epithelium with sodium hydroxide and 8 M urea solutions, by digesting it with trypsin, lecithinase C, and lecithinase D, or by treating it with lipid solvents such as Triton x 100 and saponin. From this it is concluded that the barrier depends on the integrity of lipoprotein cell membranes. The barrier function is also destroyed by sodium thioglycollate solutions, and electron microscope investigations show that sodium thioglycollate damages the thick asymmetric membrane which limits the luminal face of the superficial squamous cell. Cytochemical staining shows the epithelium to contain disulfide and thiol groups and to have a concentration of these groups at the luminal margin of the superficial cells. It thus appears that the permeability barrier also depends on the presence of disulfide bridges in the epithelium, and it is presumed that these links are located in keratin. Becau...Continue Reading

References

Jul 1, 1965·The Journal of Cell Biology·R M Hicks
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Dec 8, 1962·Nature·A D BANGHAMJ A LUCY
Feb 1, 1965·The Journal of Cell Biology·A G MATOLTSY, P F PARAKKAL

Citations

Mar 1, 1979·The American Journal of Anatomy·E R Lacy, B Schmidt-Nielsen
Aug 1, 1979·The American Journal of Anatomy·E R Lacy, B Schmidt-Nielsen
Apr 1, 1972·The Anatomical Record·J A LucasJ L Schardein
Jan 1, 1982·Anatomy and Embryology·K Tiedemann
May 3, 1979·Anatomy and Embryology·K Tiedemann
Jan 1, 1991·Cell and Tissue Research·T Inoue, G Gabella
Jan 1, 1968·Zeitschrift für Zellforschung und mikroskopische Anatomie·B Monis, D Zambrano
Jan 1, 1968·Zeitschrift für Zellforschung und mikroskopische Anatomie·B Monis, D Zambrano
Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·J G ForsbergA Norgren
Jan 1, 1967·Zeitschrift für Zellforschung und mikroskopische Anatomie·T Matsusaka
Nov 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A M RyanP D'Eustachio
Jan 1, 1967·Protoplasma·K R PorterS Badenhausen
Jan 1, 1996·Pflügers Archiv : European journal of physiology·K Jezernik
Jan 1, 1973·Zeitschrift für Zellforschung und mikroskopische Anatomie·W BreipohlM Pfautsch
May 1, 1991·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G D Heaton, A G Renwick
Sep 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J M DeSesso
Sep 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S M Cohen
Sep 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D B ClaysonS M Cohen
May 11, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·F E Hossler, Race L Kao
Sep 14, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·F E HosslerFriederike Finsterbusch
Apr 1, 1972·The Journal of Cell Biology·F J ChlapowskiL A Staehelin
May 1, 1977·The Journal of Cell Biology·J M Caruthers, M A Bonneville
Aug 1, 1980·The Journal of Cell Biology·J D Robertson, J Vergara
Jul 1, 1981·Acta Pathologica Japonica·M TatematsuN Ito
Dec 1, 1969·British Journal of Urology·D B Clayson, E H Cooper
Oct 1, 1975·British Journal of Urology·W E Harper, L R Matz
May 1, 1975·Biological Reviews of the Cambridge Philosophical Society·R M Hicks
Nov 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yanru ChenGert Kreibich
Nov 1, 1992·The American Journal of the Medical Sciences·A J KeyserE B Howard
Apr 1, 1993·The Anatomical Record·K Jezernik, N Pipan
May 1, 1995·The Anatomical Record·G C Douglas, F E Hossler
Dec 1, 1995·The Anatomical Record·F E Hossler, F C Monson
Mar 1, 1974·Kidney International·F J Silverblatt
Jan 1, 1970·Tissue & Cell·J L Oschman, M J Berridge
Sep 1, 1978·Journal of Ultrastructure Research·R C Warren, R M Hicks
Dec 28, 1969·Journal of Molecular Biology·J VergaraJ D Robertson
Apr 1, 1970·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B FlaksJ C Knowles
Jan 1, 1980·Cell and Tissue Kinetics·F A StewartD G Hirst
Oct 1, 1983·British Journal of Urology·J EldrupB Hainau
Apr 1, 1981·British Journal of Urology·K C Pringle
May 1, 1980·The Anatomical Record·J Alroy, R S Weinstein
Mar 1, 1982·The American Journal of Anatomy·R Verani, R E Bulger
Jul 20, 2002·The Journal of Urology·Lee-Fong LinAnthony Atala
Jun 22, 1973·Biochimica Et Biophysica Acta·B KettererD Beale
Jan 14, 1977·Differentiation; Research in Biological Diversity·H Zerban, W W Franke
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T MatsuzakiK Takata
Oct 1, 1977·The American Journal of Anatomy·Y C Wong, B F Martin
Jul 24, 2008·Bone Marrow Transplantation·L M BallEBMT Paediatric Working Party
Jan 1, 1990·The Journal of Urology·C L ParsonsH Callahan
Apr 6, 2000·American Journal of Physiology. Renal Physiology·J P LavelleG Apodaca
May 11, 2005·Molecular and Cellular Biochemistry·Guillermina A BongiovanniReyna O Calderón
Nov 1, 1996·International Journal of Urology : Official Journal of the Japanese Urological Association·C L Parsons
Apr 1, 1990·The Journal of Urology·C L Parsons, R E Hurst
Jul 1, 1992·The Journal of Urology·S J KalotaC L Parsons
Apr 1, 1991·The Journal of Urology·C L ParsonsP Stein
Mar 1, 1976·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·H StarklintN K Jensen
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Ping HuTung-Tien Sun

Related Concepts

Urinary Bladder
Cell Membrane Permeability
Common Bile Duct Structure
Mesothelium
Glycolate Ethers
Cytochemistry
Keratin-Associated Proteins
Electron Microscopy
Sulfhydryl Compounds
Thioglycolates

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