The permeability transition pore opening in intact mitochondria and submitochondrial particles

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
D V de MacedoL Pereira-Da-Silva

Abstract

The mitochondrial permeability transition was investigated under both oxidative and nonoxidative conditions. It was observed that dithiothreitol (DTT) was able to inhibit the permeability transition only when an oxidant, t-butylhydroperoxide, was used. Although cyclosporin A (CsA) showed also a partial protective effect under these conditions, it progressively lost its ability as the oxidant concentration was increased. Indeed, CsA and ADP were very effective under nonoxidative conditions where Ca2+ and Pi were used to induce the permeability transition, and no effect of DTT was observed. These results suggest that the Ca(2+)-dependent permeability transition pore opening is not directly dependent of dithiol oxidation. It was also shown here that CsA, independent of the presence of ADP, was able to restore the mitochondrial membrane electrical potential (delta psi) after the Ca(2+)-induced collapse. Moreover, carboxyatractyloside (CAT) did not prevent the effect of CsA, even when previously added, although it completely abolished the protective effect of ADP, indicating the participation of the ADP/ATP carrier on this process. The data with submitochondrial particles, besides providing further support to the existence of two di...Continue Reading

References

Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Apr 28, 1978·Annals of the New York Academy of Sciences·T E GunterK K Gunter
Jul 1, 1979·Archives of Biochemistry and Biophysics·D R Hunter, R A Haworth
Jan 2, 1975·Biochemical and Biophysical Research Communications·V M Madeira
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·I Szabó, M Zoratti
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·K W KinnallyD B Zorov
Jun 15, 1992·FEBS Letters·K M BroekemeierD R Pfeiffer
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Aug 1, 1993·European Journal of Biochemistry·D V de MacedoL Pereira-da-Silva

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Citations

Sep 3, 2014·Journal of Molecular and Cellular Cardiology·Andrew P Halestrap, Andrew P Richardson
Nov 17, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Reginald C AdieleCollins Kamunde
Apr 24, 2013·Parasite Immunology·R C G FaresJ A S Gomes
Jun 14, 2002·Journal of Inorganic Biochemistry·José Carlos ToledoDouglas Wagner Franco

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