The peroxisomal NAD+ carrier of Arabidopsis thaliana transports coenzyme A and its derivatives.

Journal of Bioenergetics and Biomembranes
Gennaro AgrimiFerdinando Palmieri

Abstract

The peroxisomal protein PXN encoded by the Arabidopsis gene At2g39970 has very recently been found to transport NAD+, NADH, AMP and ADP. In this work we have reinvestigated the substrate specificity and the transport properties of PXN by using a wide range of potential substrates. Heterologous expression in bacteria followed by purification, reconstitution in liposomes, and uptake and efflux experiments revealed that PNX transports coenzyme A (CoA), dephospho-CoA, acetyl-CoA and adenosine 3', 5'-phosphate (PAP), besides NAD+, NADH, AMP and ADP. PXN catalyzed fast counter-exchange of substrates and much slower uniport and was strongly inhibited by pyridoxal 5'-phosphate, bathophenanthroline and tannic acid. Transport was saturable with a submillimolar affinity for NAD+, CoA and other substrates. The physiological role of PXN is probably to provide the peroxisomes with the essential coenzymes NAD+ and CoA.

References

Oct 9, 2002·The Plant Journal : for Cell and Molecular Biology·Martin FuldaErnst Heinz
Jun 1, 2005·The Plant Cell·Itsara PracharoenwattanaSteven M Smith
Nov 18, 2005·The Journal of Biological Chemistry·Simona TodiscoFerdinando Palmieri
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marjorie J LindhurstLeslie G Biesecker
Mar 1, 2008·Molecular Membrane Biology·J Ramon De LucasFerdinando Palmieri
Nov 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alan J RobinsonEdmund R S Kunji
Dec 17, 2008·The Plant Cell·Nicole LinkaAndreas P M Weber
Jun 11, 2010·Essays in Biochemistry·Ferdinando Palmieri, Ciro Leonardo Pierri
Jun 17, 2010·The Journal of Biological Chemistry·Sreevidya AluvilaRonald S Kaplan
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Ferdinando PalmieriAlisdair R Fernie
Jul 20, 2011·The Journal of Biological Chemistry·Scott A LawrenceRichard G Moran
Sep 8, 2011·The Plant Journal : for Cell and Molecular Biology·Kristin BernhardtNicole Linka
Oct 29, 2011·Plant & Cell Physiology·Shoji ManoMikio Nishimura
Dec 22, 2011·The Biochemical Journal·Gennaro AgrimiFerdinando Palmieri

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Citations

Aug 21, 2013·Molecular Biotechnology·Giulia GiannuzziCarlo M T Marobbio
Jan 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carine De Marcos LousaFrederica L Theodoulou
Apr 18, 2013·Plant Physiology·Rémi ZallotAndrew D Hanson
Jan 22, 2013·Annual Review of Plant Biology·Lee J Sweetlove, Alisdair R Fernie
Mar 13, 2016·Biochimica Et Biophysica Acta·Ferdinando Palmieri, Magnus Monné
Sep 17, 2013·The New Phytologist·Laura-Anne BrownAlison Baker
May 24, 2015·Amino Acids·Magnus MonnéFerdinando Palmieri
Aug 31, 2013·The Biochemical Journal·Manuel Gutiérrez-Aguilar, Christopher P Baines
Nov 6, 2012·Molecular Membrane Biology·Magnus MonnéEdmund R S Kunji
Oct 30, 2012·Plant Signaling & Behavior·Pierre PétriacqBertrand Gakière
Jan 10, 2018·The Plant Journal : for Cell and Molecular Biology·Funmilayo AdebesinNatalia Dudareva
Feb 15, 2019·Journal of Integrative Plant Biology·Lennart ChartonNicole Linka
Jul 7, 2019·The Plant Journal : for Cell and Molecular Biology·Izabel de Souza ChavesAdriano Nunes-Nesi
Aug 24, 2019·The New Phytologist·Ronghui PanJianping Hu
Apr 25, 2020·The Biochemical Journal·Danielle S BritoAdriano Nunes-Nesi
Jul 25, 2019·Frontiers in Plant Science·Fan ZhangZhiwei Qin
Aug 28, 2020·Biomolecules·Adriano Nunes-NesiAlisdair R Fernie
Oct 17, 2014·The Journal of Biological Chemistry·Maria Antonietta Di NoiaFerdinando Palmieri
Jun 11, 2016·Biochemical Society Transactions·Frederica L TheodoulouAlison Baker
Aug 19, 2020·Biomolecules·Anastasija PlettNicole Linka
Aug 14, 2019·Frontiers in Plant Science·Shin-Nosuke Hashida, Maki Kawai-Yamada
Jun 18, 2020·International Journal of Molecular Sciences·Jing CuiGuillaume Tcherkez
Dec 1, 2020·Plant Physiology and Biochemistry : PPB·Vincenzo TragniCiro Leonardo Pierri
Oct 1, 2020·The Plant Journal : for Cell and Molecular Biology·Elias Feitosa-AraujoAdriano Nunes-Nesi
Jul 9, 2020·Synthetic and Systems Biotechnology·Xuan CaoYongjin J Zhou
May 1, 2021·Plants·Franziska KuhnertMarion Eisenhut
Sep 21, 2021·The EMBO Journal·Sergio Guerrero-CastilloSusanne Arnold

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