The Peroxygenase Activity of the Aspergillus flavus Caleosin, AfPXG, Modulates the Biosynthesis of Aflatoxins and Their Trafficking and Extracellular Secretion via Lipid Droplets

Frontiers in Microbiology
Abdulsamie HananoDenis J Murphy

Abstract

Aflatoxins (AF) are highly detrimental to human and animal health. We recently demonstrated that the Aspergillus flavus caleosin, AfPXG, had peroxygenase activity and mediated fungal development and AF accumulation. We now report the characterization of an AfPXG-deficient line using reference strain NRRL3357. The resulting fungal phenotype included a severe decrease in mycelium growth, failure to sporulate, and reduced AF production. Increasing cellular oxidative status by administration of hydrogen peroxide and cumene hydroperoxide did not restore the AfPXG-deficient phenotype, which suggests that AfPXG-deficiency is not directly related to oxidative stress. To investigate possible alternative roles of AfPXG, a gain of function approach was used to overexpress AfPXG, with the reporter gene Gfp, in an AfPXG-deficient line, termed AfPXG+ . The resulting phenotype included elevated numbers of stable lipid droplets (LDs) plus enhanced AF production. Highly purified LDs from AfPXG+ cultures sequestered AF and this ability was positively correlated with overall LD number. Site-specific mutagenesis of AfPXG to delete Histidine 85 (AfPXGHis85), a residue essential for its catalytic activity, or deletion of the putative LD targeting do...Continue Reading

References

Sep 1, 1989·Mycopathologia·C Fanelli, A A Fabbri
Nov 21, 2000·Free Radical Biology & Medicine·T Jayashree, C Subramanyam
Feb 24, 2001·Plant Molecular Biology·H NaestedJ Mundy
May 16, 2001·The Journal of Biological Chemistry·A M CalvoN P Keller
Dec 31, 2003·The Journal of Biological Chemistry·Dimitrios I TsitsigiannisNancy P Keller
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Dec 23, 2004·Systematic and Applied Microbiology·Jens C Frisvad, Robert A Samson
Sep 2, 2005·Molecular Plant-microbe Interactions : MPMI·Lori A Maggio-HallNancy P Keller
Dec 3, 2005·Nature Reviews. Microbiology·Nancy P KellerJoan W Bennett
Dec 24, 2005·Nature·Masayuki MachidaHisashi Kikuchi
Sep 8, 2006·The Journal of Biological Chemistry·Abdulsamie HananoElizabeth Blée
Oct 20, 2006·Nature·Timothy Y JamesRytas Vilgalys
Feb 6, 2007·Trends in Microbiology·Dimitrios I Tsitsigiannis, Nancy P Keller
Jun 2, 2007·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takeshi AkaoOsamu Akita
Sep 25, 2007·Applied and Environmental Microbiology·Ludmila V RozeJohn E Linz
Oct 19, 2007·Chemosphere·J BoucherI W Marison
Jan 10, 2008·Molecular Microbiology·Marion BrodhagenNancy P Keller
Feb 21, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·G S Shephard
Jul 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jordan L CocchiaroRaphael H Valdivia
Jul 10, 2008·The Journal of Biological Chemistry·Joel M Goodman
Feb 18, 2009·FEMS Yeast Research·Marine FroissardThierry Chardot
Mar 12, 2009·The Journal of Chemical Physics·Zilu Wang, Xuehao He
May 27, 2009·Plant Physiology and Biochemistry : PPB·Mark Partridge, Denis J Murphy
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anindya ChandaJohn E Linz
May 11, 2010·Applied and Environmental Microbiology·José Miguel P Ferreira de OliveiraLeo H de Graaff
May 25, 2010·Applied Microbiology and Biotechnology·Martin HofrichterTaina Lundell
Mar 1, 2005·Molecular Plant Pathology·Stephen C WhissonJohn T Jones
Sep 21, 2010·Applied Biochemistry and Biotechnology·Stefania BonsegnaAngelo Santino
Sep 28, 2010·Eukaryotic Cell·Anindya ChandaJohn E Linz
Feb 2, 2011·The FEBS Journal·Florian Brodhun, Ivo Feussner
Aug 5, 2011·The FEBS Journal·Sebastian PeterMartin Hofrichter
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·William C RatcliffMichael Travisano
Oct 25, 2012·PloS One·Massimo ReverberiCorrado Fanelli
Sep 24, 2013·Current Genetics·Maja RadulovicSepp D Kohlwein

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Methods Mentioned

BETA
gene knockouts
PCR
transfection
fluorescence microscopy
Fluorescence

Software Mentioned

CLCBio
BLAST
WebScipio
Rasmol
CLC viewer
Clustal Omega

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