The PGL family proteins associate with germ granules and function redundantly in Caenorhabditis elegans germline development

Genetics
Ichiro KawasakiSusan Strome

Abstract

PGL-1 is a constitutive protein component of C. elegans germ granules, also known as P granules. Maternally supplied PGL-1 is essential for germline development but only at elevated temperature, raising the possibility that redundant factors provide sufficient function at lower temperatures. We have identified two PGL-1-related proteins, PGL-2 and PGL-3, by sequence analysis of the C. elegans genome and by a yeast two-hybrid screen for proteins that interact with PGL-1. PGL-3 is associated with P granules at all stages of development, while PGL-2 is associated with P granules only during postembryonic development. All three PGL proteins interact with each other in vitro. Furthermore, PGL-1 and PGL-3 are co-immunoprecipitated from embryo extracts, indicating that they are indeed in the same protein complex in vivo. Nevertheless, each PGL protein localizes to P granules independently of the other two. pgl-2 or pgl-3 single-mutant worms do not show obvious defects in germline development. However, pgl-1; pgl-3 (but not pgl-2; pgl-1) double-mutant hermaphrodites and males show significantly enhanced sterility at all temperatures, compared to pgl-1 alone. Mutant hermaphrodites show defects in germline proliferation and in production...Continue Reading

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Citations

Jun 10, 2010·Development Genes and Evolution·Luis A Bezares-CalderónRosa E Navarro
Dec 24, 2010·Genome Research·W Clay SpencerDavid M Miller
Feb 1, 2008·Development·Caroline A SpikeSusan Strome
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Mar 16, 2016·Proceedings of the National Academy of Sciences of the United States of America·Andrew Kekūpa'a KnutsonSusan Strome
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May 9, 2006·Developmental Biology·Eri HasegawaMasayuki Yamamoto

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