The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.

Journal of Molecular Biology
Aleksandra A WatsonErnest D Laue

Abstract

The NuRD (nucleosome remodeling and deacetylase) complex serves as a crucial epigenetic regulator of cell differentiation, proliferation, and hematopoietic development by coupling the deacetylation and demethylation of histones, nucleosome mobilization, and the recruitment of transcription factors. The core nucleosome remodeling function of the mammalian NuRD complex is executed by the helicase-domain-containing ATPase CHD4 (Mi-2β) subunit, which also contains N-terminal plant homeodomain (PHD) and chromo domains. The mode of regulation of chromatin remodeling by CHD4 is not well understood, nor is the role of its PHD and chromo domains. Here, we use small-angle X-ray scattering, nucleosome binding ATPase and remodeling assays, limited proteolysis, cross-linking, and tandem mass spectrometry to propose a three-dimensional structural model describing the overall shape and domain interactions of CHD4 and discuss the relevance of these for regulating the remodeling of chromatin by the NuRD complex.

References

Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Jun 1, 2005·Biophysical Journal·Maxim V Petoukhov, Dmitri I Svergun
Nov 22, 2005·Trends in Biochemical Sciences·Mariann Bienz
Jan 24, 2006·Molecular and Cellular Biology·Xavier Le GuezennecHendrik G Stunnenberg
Apr 12, 2006·The Journal of Biological Chemistry·Chris StockdaleTom Owen-Hughes
Jun 2, 2006·Nucleic Acids Research·Andrew FlausTom Owen-Hughes
Jun 13, 2006·Structure·Maxim V PetoukhovDmitri I Svergun
Aug 19, 2007·Oncogene·S A Denslow, P A Wade
Feb 23, 2008·Journal of Molecular Biology·Mark YoulesErmanno Gherardi
Sep 9, 2008·The International Journal of Biochemistry & Cell Biology·Patrick McDonelBrian Hendrich
Oct 22, 2008·Current Opinion in Genetics & Development·Vincent T Cunliffe
Jan 20, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ashley C W PikeOpher Gileadi
Apr 10, 2009·Annual Review of Biochemistry·Cedric R Clapier, Bradley R Cairns
Jun 19, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Natascha Kunert, Alexander Brehm
Jul 25, 2009·The Biochemical Journal·Catherine A MusselmanTatiana G Kutateladze
Jun 15, 2010·Journal of Structural Biology·Pavel SavitskyOpher Gileadi
Aug 25, 2010·Molecular and Cellular Biology·B Ashok ReddyC Peter Verrijzer
Jan 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Catherine A MusselmanTatiana G Kutateladze

❮ Previous
Next ❯

Citations

Sep 7, 2013·Nature Structural & Molecular Biology·Felix Mueller-PlanitzPeter B Becker
Oct 17, 2012·Molecular and Cellular Biology·Julita RamírezJames Hagman
Mar 19, 2013·PLoS Genetics·Gundula StreubelUta-Maria Bauer
Jan 24, 2013·Cellular and Molecular Life Sciences : CMLS·Hillary F AllenTatiana G Kutateladze
Oct 7, 2014·Expert Opinion on Biological Therapy·Gun-Il Im, Kyung-Ju Shin
Jan 23, 2016·Journal of Medicinal Chemistry·Vineet Pande
Aug 16, 2014·Immunological Reviews·Carissa Dege, James Hagman
Jun 1, 2014·Translational Research : the Journal of Laboratory and Clinical Medicine·Jeannine Basta, Michael Rauchman
Jun 8, 2015·Trends in Genetics : TIG·Stefania BottardiEric Milot
May 24, 2013·Biochemical Society Transactions·Aoife O'Shaughnessy, Brian Hendrich
Jun 16, 2015·Genes·Gernot Längst, Laura Manelyte
Mar 23, 2015·Cellular and Molecular Life Sciences : CMLS·Morgan P TorchyBruno P Klaholz
Jun 28, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Da-Qiang LiRakesh Kumar
Aug 6, 2013·Cell·Geeta J NarlikarTom Owen-Hughes
Sep 7, 2016·Journal of Molecular Biology·Biswaranjan MohantyDaniel P Ryan
Sep 23, 2016·Cell Reports·Forest H AndrewsTatiana G Kutateladze
Oct 13, 2016·Frontiers in Molecular Biosciences·Rita PuglisiAnnalisa Pastore
May 18, 2017·Nature Reviews. Molecular Cell Biology·Cedric R ClapierCraig L Peterson
Jul 15, 2017·Nature Communications·Emma A MorrisonCatherine A Musselman
Nov 16, 2017·Nature Communications·Jovylyn GatchalianTatiana G Kutateladze
Mar 26, 2014·Molecular and Cellular Biology·Kevin M CreamerJanet F Partridge
Jan 13, 2019·The FEBS Journal·Masooma RasheedAnnalisa Pastore
Jun 13, 2014·The Journal of Biological Chemistry·Saad S M AlqarniErnest D Laue
Feb 20, 2018·Frontiers in Molecular Biosciences·Salvatore AdinolfiAnnalisa Pastore
Aug 8, 2019·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Karin WeissKatherine Lachlan
Mar 16, 2019·Scientific Reports·Lisa D McKenzieMilan G Chheda
Jan 10, 2021·International Journal of Molecular Sciences·Poyil PratheeshkumarKhawla S Al-Kuraya
Sep 17, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Vijaya Lakshmi MalisettyBramanandam Manavathi
May 6, 2021·International Journal of Molecular Sciences·Sarah LarriganPierre Mattar
May 14, 2021·Frontiers in Oncology·Apolonia NovilloPablo Gómez-Del Arco

❮ Previous
Next ❯

Methods Mentioned

BETA
size-exclusion chromatography
dot blots
X-ray
dynamic light scattering
reverse-phase chromatography

Software Mentioned

MASCOT
DAMCLUST
CORAL COmplexes
GraphPad Prism
AIDA
MODEL
MONSA
CORAL
DAMMIF
SWISS

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.