The PHD-finger module of the Arabidopsis thaliana defense regulator EDM2 can recognize triply modified histone H3 peptides

Plant Signaling & Behavior
Tokuji Tsuchiya, Thomas Eulgem

Abstract

Recently we reported that the Arabidopsis thaliana PHD-finger protein EDM2 (enhanced downy mildew 2) impacts disease resistance by affecting levels of di-methylated lysine 9 of histone H3 (H3K9me2) at an alternative polyadenylation site in the immune receptor gene RPP7. EDM2-dependent modulation of this post-translational histone modification (PHM) shifts the balance between full-length RPP7 transcripts and prematurely polyadenylated transcripts, which do not encode the RPP7 protein. Our previous work genetically linked, for the first time, PHMs to alternative polyadenylation and established EDM2 as a critical component mediating PHM-dependent polyadenylation control. However, how EDM2 is recruited to its genomic target sites and how it affects H3K9me2 levels is unknown. Here we show the PHD-finger module of EDM2 to recognize histone H3 bearing certain combinations of 3 distinct PHMs. Our results suggest that targeting of EDM2 to specific genomic regions is mediated by the histone-binding selectivity of its PHD-finger domain.

References

Aug 11, 2001·Science·T Jenuwein, C D Allis
May 27, 2006·Nature·Pedro V PeñaTatiana G Kutateladze
Jan 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas EulgemJeffery L Dangl
Jan 22, 2009·The Plant Journal : for Cell and Molecular Biology·Woo Yong LeeChang Seob Kwon
Oct 24, 2009·Trends in Genetics : TIG·François RoudierVincent Colot
Feb 13, 2010·The Plant Journal : for Cell and Molecular Biology·Tokuji Tsuchiya, Thomas Eulgem
Sep 4, 2010·Cell·Jung-Shin LeeAli Shilatifard
Sep 16, 2010·BMC Plant Biology·Tokuji Tsuchiya, Thomas Eulgem
Mar 5, 2011·The Journal of Hand Surgery, European Volume·R C TakemotoK A Egol
Aug 13, 2011·Molecular Plant-microbe Interactions : MPMI·Tokuji Tsuchiya, Thomas Eulgem
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tokuji Tsuchiya, Thomas Eulgem
Sep 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·John M McDowell, Blake C Meyers
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mingguang LeiJian-Kang Zhu
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Liuyin MaQingshun Quinn Li

❮ Previous
Next ❯

Citations

Aug 6, 2016·Annual Review of Phytopathology·A DelerisL Navarro
Aug 27, 2016·Frontiers in Plant Science·Nino A EspinasYusuke Saijo
Aug 24, 2017·Molecular Plant Pathology·Yan Lai, Thomas Eulgem
Mar 25, 2017·Scientific Reports·Kenji OsabeHidetoshi Saze
Jun 12, 2016·The Biochemical Journal·Yanli Liu, Jinrong Min
Jan 14, 2021·Journal of Integrative Plant Biology·Yi-Zhe ZhangCheng-Guo Duan
Jul 6, 2015·Plant Physiology·Taiko K ToTetsuji Kakutani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Tokuji Tsuchiya, Thomas Eulgem
The Plant Journal : for Cell and Molecular Biology
Tokuji Tsuchiya, Thomas Eulgem
Proceedings of the National Academy of Sciences of the United States of America
Mingguang LeiJian-Kang Zhu
© 2021 Meta ULC. All rights reserved