Abstract
Cultured maize cells habituated to grow in the presence of the cellulose synthesis inhibitor dichlobenil (DCB) have a modified cell wall in which the amounts of cellulose are reduced and the amounts of arabinoxylan increased. This paper examines the contribution of cell wall-esterified hydroxycinnamates to the mechanism of DCB habituation. For this purpose, differences in the phenolic composition of DCB-habituated and non-habituated cell walls, throughout the cell culture cycle and the habituation process were characterized by HPLC. DCB habituation was accompanied by a net enrichment in cell wall phenolics irrespective of the cell culture phase. The amount of monomeric phenolics was 2-fold higher in habituated cell walls. Moreover, habituated cell walls were notably enriched in p-coumaric acid. Dehydrodimers were 5-6-fold enhanced as a result of DCB habituation and the steep increase in 8,5'-diferulic acid in habituated cell walls would suggest that this dehydrodimer plays a role in DCB habituation. In summary, the results obtained indicate that cell wall phenolics increased as a consequence of DCB habituation, and suggest that they would play a role in maintaining the functionality of a cellulose impoverished cell wall.
Citations
Dec 18, 2013·Journal of Plant Physiology·María de CastroJosé Luis Acebes
Oct 11, 2011·Applied and Environmental Microbiology·Lysiane DunièreDelphine Thévenot-Sergentet
Nov 22, 2015·Journal of Experimental Botany·Mizuki TatenoSeth DeBolt
Jan 27, 2012·Physiologia Plantarum·Zara MeraliKeith W Waldron
Mar 5, 2015·Journal of Integrative Plant Biology·Hugo MélidaAntonio Encina
May 17, 2011·Molecular Plant·Hugo MélidaStephen C Fry
Jul 28, 2011·Plant Signaling & Behavior·Ana Alonso-SimónJosé L Acebes
May 6, 2017·Journal of Integrative Plant Biology·María de CastroPenélope García-Angulo
Feb 22, 2018·Physiologia Plantarum·Romina Martínez-RubioAnna Kärkönen
Jan 23, 2015·Journal of Integrative Plant Biology·María de CastroStephen C Fry
Nov 28, 2015·Physiologia Plantarum·Asier Largo-GosensJesús M Álvarez
Jun 22, 2018·Genes·Gea GuerrieroMohammad Faisal
Jan 21, 2011·Plant Signaling & Behavior·Hugo MélidaAntonio Encina
Aug 1, 2014·Plant Physiology·Chad BrabhamSeth DeBolt