The phenotypic characteristic of liver-derived stem cells from adult human deceased donor liver

Transplantation Proceedings
J-H LeeS-K Lee

Abstract

Liver transplantation is the only effective treatment for end-stage liver disease. Because of the limited donor availability, attention has been focused on the possibility to restore liver mass and function through cell transplantation. Stem cells are a promising source for liver repopulation after cell transplantation, but whether or not the adult liver contains hepatic stem cells is highly controversial. Several studies have suggested the presence of stem cells in the adult normal human liver. However, a population with stem cell properties has not yet been isolated. The purpose of this study was to identify and characterize progenitor cells in normal adult human liver. We isolated and expanded human liver stem cells (HLSCs) from a donated liver not suitable for liver transplantation or characterizing them by fluorescence-activated cell sorter, polymerase chain reaction, and immunofluorescence assay. HLSCs expressed the mesenchymal stem cell markers CD29, CD73, CD44, CD90, CD105, and CD166 but not the hematopoietic stem cell markers CD34, CD45, and CD117. HLSCs were also positive for vimentin and nestin, a stem cell marker. The absence of staining for cytokeratin-19, CD117, and CD34 indicated that HLSCs were not oval stem cel...Continue Reading

References

Mar 9, 2006·American Journal of Obstetrics and Gynecology·C Bettina Portmann-LanzDaniel V Surbek
Sep 2, 2006·Stem Cells·Maria Beatriz HerreraGiovanni Camussi
Oct 5, 2007·Cell Transplantation·Kwang-Woong LeeSuk-Koo Lee

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Citations

Aug 29, 2014·Cellular Oncology (Dordrecht)·Ekaterini Christina TampakiGregory Kouraklis
Apr 25, 2013·World Journal of Gastroenterology : WJG·Minoru ItouMichio Sata
May 14, 2016·Stem Cell Research & Therapy·Yini WangLanuan Li
Sep 25, 2019·Cells·Irina V KholodenkoKonstantin N Yarygin
Nov 20, 2017·Tissue Engineering and Regenerative Medicine·Dongkyu KimJong-Hoon Kim
May 14, 2021·Frontiers in Cell and Developmental Biology·Stefania BrunoGiovanni Camussi

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