The phloem-limited bacterium of greening disease of citrus is a member of the alpha subdivision of the Proteobacteria

International Journal of Systematic Bacteriology
S JagoueixM Garnier

Abstract

Using the PCR, we amplified the 16S ribosomal DNAs (rDNAs) of an Asian strain and an African strain of the uncultured, gram-negative, walled, phloem-limited bacterium-like organism (BLO) associated with citrus greening disease. We evaded coamplification of chloroplast 16S rDNA by using restriction enzymes; the chloroplast 16S rDNA was sensitive to BclI digestion and resistant to EcoRI digestion, while the 16S rDNA of the BLO was resistant to BclI digestion and sensitive to EcoRI digestion. The 16S rDNA of the African BLO strain was amplified from BclI-digested DNA extracted from infected periwinkle leaf midribs. The Asian strain was isolated from plant extract by using a specific monoclonal antibody coated onto the surface of a PCR tube. The 16S rDNAs of the two BLO strains were cloned and sequenced. Comparisons with sequences of 16S rDNAs obtained from the GenBank data base revealed that the two citrus greening disease BLOs belong to the alpha subdivision of the class Proteobacteria. Even though their closest relatives are members of the alpha-2 subgroup, these BLOs are distinct from this subgroup as we observed only 87.5% homology between the 16S rDNAs examined. Therefore, the two BLOs which we studied probably are members of...Continue Reading

Citations

Aug 15, 2001·Folia Microbiologica·L KlucarJ Kormanec
May 8, 2010·Applied Microbiology and Biotechnology·Adrián Alberto VojnovMarìa Rosa Marano
Jan 15, 2013·Antonie van Leeuwenhoek·Man-Young JungSung-Keun Rhee
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·S Jagoueix-EveillardM Garnier
Feb 28, 2013·Phytopathology·Nian Wang, Pankaj Trivedi
Nov 5, 2005·Applied and Environmental Microbiology·C BastianelS Eveillard
Feb 15, 2013·Standards in Genomic Sciences·Michael T LeonardEric W Triplett
Nov 10, 2013·Molecular Plant-microbe Interactions : MPMI·Nelson A WulffDean W Gabriel

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