The PHO signaling pathway directs lipid remodeling in Cryptococcus neoformans via DGTS synthase to recycle phosphate during phosphate deficiency

PloS One
Sophie LevJulianne T Djordjevic

Abstract

The phosphate sensing and acquisition (PHO) pathway of Cryptococcus neoformans is essential for growth in phosphate-limiting conditions and for dissemination of infection in a mouse model. Its key transcription factor, Pho4, regulates expression of genes controlling the acquisition of phosphate from both external and cellular sources. One such gene, BTA1, is highly up-regulated during phosphate starvation. Given that a significant proportion of cellular phosphate is incorporated into phospholipids, and that the Pho4-dependent BTA1 gene encodes an enzyme predicted to catalyse production of a phosphorus-free betaine lipid, we investigated whether phospholipids provide an accessible reservoir of phosphate during phosphate deficiency. By comparing lipid profiles of phosphate-starved WT C. neoformans, PHO4 (pho4Δ) and BTA1 (bta1Δ) deletion mutants using thin layer chromatography and liquid chromatography mass spectrometry, we showed that phosphatidylcholine (PC) is substituted by the phosphorus-free betaine lipids diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) and diacylgyceryl hydroxymethyl-N,N,N-trimethyl-beta-alanine (DGTA) in a Pho4- and Bta1-dependent manner, and that BTA1 encodes a functional DGTS synthase. Synthesis of DGTA ...Continue Reading

References

Mar 1, 1993·Journal of Bacteriology·D L ToffalettiJ R Perfect
Mar 1, 1996·Progress in Lipid Research·V M Dembitsky
Dec 31, 1997·Phytochemistry·K Künzler, W Eichenberger
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R M Klug, C Benning
Jun 20, 2008·The Plant Journal : for Cell and Molecular Biology·Nicole GaudePeter Dörmann
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maritza Zavaleta-PastorOtto Geiger
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cyrille Y BottéGeoffrey I McFadden
Apr 9, 2014·Infection and Immunity·Matthias KretschmerJames W Kronstad
May 23, 2014·Journal of Biosciences·Parul Tomar, Himanshu Sinha
Apr 10, 2015·PloS One·Samantha D M ArrasJames A Fraser
May 10, 2015·Fungal Genetics and Biology : FG & B·Akio Toh-eMasafumi Nishizawa
Mar 31, 2016·Sub-cellular Biochemistry·Barbara KalischGeorg Hölzl
May 10, 2017·The Lancet Infectious Diseases·Radha RajasinghamDavid R Boulware
Aug 16, 2017·Journal of Lipid Research·Ashutosh SinghMaurizio Del Poeta

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Citations

Aug 8, 2020·Frontiers in Cellular and Infection Microbiology·Adriana Marcela Celis RamírezHans de Cock
Mar 16, 2021·Cellular Microbiology·Sophie LevJulianne Teresa Djordjevic
Sep 5, 2021·Trends in Microbiology·Kabir BhallaJames W Kronstad

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Methods Mentioned

BETA
PCR
deacylation

Software Mentioned

DGTS
Agilent MassHunter quantitative
DGTA

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