The phosphatidylinositol synthase gene (GhPIS) contributes to longer, stronger, and finer fibers in cotton

Molecular Genetics and Genomics : MGG
Qin LongYan Pei

Abstract

Cotton fibers are the most important natural raw material used in textile industries world-wide. Fiber length, strength, and fineness are the three major traits which determine the quality and economic value of cotton. It is known that exogenous application of phosphatidylinositols (PtdIns), important structural phospholipids, can promote cotton fiber elongation. Here, we sought to increase the in planta production of PtdIns to improve fiber traits. Transgenic cotton plants were generated in which the expression of a cotton phosphatidylinositol synthase gene (i.e., GhPIS) was controlled by the fiber-specific SCFP promoter element, resulting in the specific up-regulation of GhPIS during cotton fiber development. We demonstrate that PtdIns content was significantly enhanced in transgenic cotton fibers and the elevated level of PtdIns stimulated the expression of genes involved in PtdIns phosphorylation as well as promoting lignin/lignin-like phenolic biosynthesis. Fiber length, strength and fineness were also improved in the transgenic plants as compared to the wild-type cotton, with no loss in overall fiber yield. Our data indicate that fiber-specific up-regulation of PtdIns synthesis is a promising strategy for cotton fiber qua...Continue Reading

References

Sep 1, 1984·American Journal of Medical Genetics·P SteinbachH Schmidt
Sep 21, 2002·Molecular Genetics and Genomics : MGG·S E HarmerJ N Timmis
Jan 7, 2003·Plant & Cell Physiology·Daniel J CosgroveDouglas Blecker
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Sep 25, 2003·Annual Review of Plant Biology·Wout BoerjanMarie Baucher
Jun 17, 2005·Plant & Cell Physiology·Yan SunRandy D Allen
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Ming LuoYan Pei
Oct 2, 2007·Annals of Botany·Jinsuk J LeeZ Jeffrey Chen
Apr 19, 2008·Plant, Cell & Environment·Gabriela GonorazkyLaura de la Canal
Sep 13, 2008·The Plant Journal : for Cell and Molecular Biology·Joop E M VermeerTheodorus W J Gadella
Jan 27, 2011·The Plant Journal : for Cell and Molecular Biology·Till IschebeckIngo Heilmann
Oct 5, 2014·Biochimica Et Biophysica Acta·Mareike Heilmann, Ingo Heilmann
Jun 16, 2016·Development·Ingo Heilmann

❮ Previous
Next ❯

Datasets Mentioned

BETA
KJ769104.1

Methods Mentioned

BETA
transgenic
phosphotransferase
PCR
transmission electron microscopy
PCRs
Scanning electron microscopy

Software Mentioned

Pro ® Plus
Image
Image J
CFX Manager

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.