The phosphoinositide phosphatase Sac1 regulates cell shape and microtubule stability in the developing Drosophila eye

Development
Lauren M Del BelJulie A Brill

Abstract

Epithelial patterning in the developing Drosophila melanogaster eye requires the Neph1 homolog Roughest (Rst), an immunoglobulin family cell surface adhesion molecule expressed in interommatidial cells (IOCs). Here, using a novel temperature-sensitive (ts) allele, we show that the phosphoinositide phosphatase Sac1 is also required for IOC patterning. Sac1ts mutants have rough eyes and retinal patterning defects that resemble rst mutants. Sac1ts retinas exhibit elevated levels of phosphatidylinositol 4-phosphate (PI4P), consistent with the role of Sac1 as a PI4P phosphatase. Indeed, genetic rescue and interaction experiments reveal that restriction of PI4P levels by Sac1 is crucial for normal eye development. Rst is delivered to the cell surface in Sac1ts mutants. However, Sac1ts mutant IOCs exhibit severe defects in microtubule organization, associated with accumulation of Rst and the exocyst subunit Sec8 in enlarged intracellular vesicles upon cold fixation ex vivo Together, our data reveal a novel requirement for Sac1 in promoting microtubule stability and suggest that Rst trafficking occurs in a microtubule- and exocyst-dependent manner.

References

Dec 1, 1989·Developmental Biology·R L Cagan, D F Ready
Apr 1, 1989·Genetics·P NovickD Botstein
Dec 1, 1989·The Journal of Cell Biology·A E ClevesV A Bankaitis
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·V RuotsalainenK Tryggvason
Sep 6, 2000·The EMBO Journal·D J GilloolyH Stenmark
Apr 28, 2001·Cell Death and Differentiation·S M GorskiR L Cagan
Jun 21, 2001·Molecular and Cellular Biology·D B DonovielD R Powell
Oct 7, 2003·The Journal of Biological Chemistry·Holger M RohdeGuido Boehmelt
Dec 10, 2003·Genome Génome / Conseil National De Recherches Canada·Ho-Chun WeiJames V Price
Apr 27, 2004·Nature Cell Biology·Anna GodiM Antonietta De Matteis
Jul 24, 2004·The Journal of Biological Chemistry·Anjana Roy, Timothy P Levine
May 18, 2005·The Journal of Cell Biology·Slobodan BeronjaUlrich Tepass
Dec 16, 2005·Development·Eric MaroisSuzanne Eaton
Apr 5, 2007·Nature Protocols·Rhian F Walther, Franck Pichaud
Feb 27, 2008·The Journal of Cell Biology·Anastasia BlagoveshchenskayaPeter Mayinger
Jun 19, 2009·Advances in Enzyme Regulation·Yang LiuVytas A Bankaitis
Sep 16, 2009·PloS One·Ruth I Johnson, Ross L Cagan
Mar 20, 2010·Molecular Biology of the Cell·Philip GoldbachJulie A Brill

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Citations

Nov 11, 2019·G3 : Genes - Genomes - Genetics·Vishnu JanardanPadinjat Raghu
Mar 19, 2020·Molecular Biology of the Cell·Nigel W GriffithsJulie A Brill

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