The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex.

The Journal of Cell Biology
Marie E BechlerWilliam J Brown

Abstract

We report that platelet-activating factor acetylhydrolase (PAFAH) Ib, comprised of two phospholipase A(2) (PLA(2)) subunits, alpha1 and alpha2, and a third subunit, the dynein regulator lissencephaly 1 (LIS1), mediates the structure and function of the Golgi complex. Both alpha1 and alpha2 partially localize on Golgi membranes, and purified catalytically active, but not inactive alpha1 and alpha2 induce Golgi membrane tubule formation in a reconstitution system. Overexpression of wild-type or mutant alpha1 or alpha2 revealed that both PLA(2) activity and LIS1 are important for maintaining Golgi structure. Knockdown of PAFAH Ib subunits fragments the Golgi complex, inhibits tubule-mediated reassembly of intact Golgi ribbons, and slows secretion of cargo. Our results demonstrate a cooperative interplay between the PLA(2) activity of alpha1 and alpha2 with LIS1 to facilitate the functional organization of the Golgi complex, thereby suggesting a model that links phospholipid remodeling and membrane tubulation to dynein-dependent transport.

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Citations

Jul 3, 2013·Histochemistry and Cell Biology·Emma Martínez-AlonsoJosé A Martínez-Menárguez
May 20, 2011·Molecular Biology of the Cell·Marie E BechlerWilliam J Brown
Aug 31, 2011·Cold Spring Harbor Perspectives in Biology·Benjamin S Glick, Alberto Luini
Nov 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jai P Pandey, Deanna S Smith
Sep 27, 2014·Experimental Cell Research·Jeerawat SoonthornsitNobuhiro Nakamura
Jun 26, 2012·Current Opinion in Cell Biology·Nobuhiro NakamuraJoachim Seemann
May 9, 2012·Biochimica Et Biophysica Acta·Kevin D HaWilliam J Brown
Dec 2, 2011·Trends in Cell Biology·Marie E BechlerWilliam J Brown
Sep 29, 2011·Cytoskeleton·Mathieu ChansardMinh Dang Nguyen
Apr 24, 2012·The Journal of Comparative Neurology·Teresa EscamezDiego Echevarria
Dec 25, 2012·Biochimica Et Biophysica Acta·Marie E Bechler, William J Brown
Sep 23, 2011·Biochemical Society Transactions·Victoria J Allan
Oct 7, 2017·Biology of the Cell·Beata KaczmarekCatherine L Jackson
Dec 21, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Xiaochao WeiClay F Semenkovich
Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Anne C S FernandesThaïs Souto-Padrón
Jan 15, 2014·Journal of Cell Science·Mariagrazia CapestranoRoman S Polishchuk

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