PMID: 7012615Feb 1, 1981Paper

The phospholipases of pathogenic and non-pathogenic Trypanosoma species

Molecular and Biochemical Parasitology
P N HambreyI R Tizard

Abstract

Four species of trypanosome were examined for phospholipase activities using 1-[3H]palmitoyl-2-acyl-sn-glycero-3-phosphocholine and 1-acyl-2[14C]linoleoyl-sn-glycero-3-phosphocholine as substrates. The major activity in each species is a phospholipase A1 (EC 3.1.1.32) which does not require calcium. The most effective of the detergents tested for activation of the enzyme from each species, and the Ph optima, are as follows: Trypanosoma brucei, 0.125% Triton X-100 at pH 6.0-8.5; T. congolense, 0.5 mM linoleate at pH 6.0; T. theileri, 0.1% Triton X-100 at pH 6.75; T. lewisi, 0.2 mM sodium dodecyl sulfate at pH 5.2. The specific activity of the enzyme from a pathogenic species, T. brucei, is very high (145 nmol/min/mg/protein) and could contribute to the tissue damage characteristically caused by this parasite. The level in T. lewisi, a non-pathogenic species, is relatively low (1 nmol/min/mg). The levels in T. theileri (31 nmol/min/mg) and T. congolense (10 nmol/min/mg are intermediate. These results are compatible with the hypothesis that phospholipases contribute to the pathogenicity of trypanosomes.

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Citations

May 1, 1982·Molecular and Biochemical Parasitology·F R Opperdoes, J van Roy
Jun 1, 1988·Molecular and Biochemical Parasitology·A SamadA Mellors
Aug 1, 1989·Parasitology Today·A Mellors, A Samad
Dec 13, 2000·Microbes and Infection·H TabelJ E Uzonna
Feb 23, 2011·International Journal of Molecular Sciences·Gregory S Richmond, Terry K Smith
Jan 24, 2007·Molecular Microbiology·Gregory S Richmond, Terry K Smith
Oct 8, 2008·Immunological Reviews·Henry TabelMeiqing Shi
Jan 1, 2013·Molecular and Biochemical Parasitology·María Laura BelaunzaránElvira Luisa Durante de Isola
May 24, 2011·Enzyme Research·María Laura BelaunzaránElvira Luisa Durante de Isola
Oct 1, 1983·Infection and Immunity·G L OtulakowskiB N Wilkie

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