The phosphoryl transfer domain of UhpB interacts with the response regulator UhpA

Journal of Bacteriology
J S Wright, R J Kadner

Abstract

Bacterial two-component regulatory systems control the expression of target genes through regulated changes in protein phosphorylation. Signal reception alters the ability of a membrane-bound histidine kinase (HK) protein to transfer phosphate from ATP to a highly conserved histidine residue. The transfer of phosphate from the histidine to an aspartate residue on the cognate response regulator (RR) changes the ability of the latter protein to bind to target DNA sequences and to alter gene transcription. UhpB is the HK protein which controls production of the sugar phosphate transporter UhpT. Elevated expression of full-length UhpB or of a soluble hybrid protein, GST-Bc, which is glutathione S-transferase (GST) fused to the cytoplasmic C-terminal portion of UhpB, results in complete blockage of uhpT expression in a uhp(+) strain. This dominant-negative interference could result from the ability of GST-Bc to bind and sequester the RR UhpA and to accelerate its dephosphorylation. The portion of GST-Bc responsible for the interference phenotype was localized using truncation, linker insertion, and point mutations to the region between residues 293 and 366 flanking His-313, the putative site of autophosphorylation. Point mutations w...Continue Reading

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Citations

Mar 26, 2003·European Journal of Biochemistry·Christian SchwöppeH Ekkehard Neuhaus
Jul 11, 2002·Journal of Bacteriology·Daniël T VerhammeKlaas J Hellingwerf
May 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Livnat LopianOrna Amster-Choder
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·S Heeb, D Haas
Aug 28, 2009·Molecular Microbiology·Larissa Tetsch, Kirsten Jung
Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, Milton H Saier
Dec 26, 2001·The Journal of Biological Chemistry·Gholson J LyonTom W Muir
Apr 19, 2003·The Journal of Biological Chemistry·María E CastelliEleonora García Vescovi

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